Abstract In this work, a chemical data collections is regarding the synthesis, purification and characterization of water-soluble fluorescent probes based on (5)6-carboxyfluorescein (CF)-conjugated with normal and reverse triblock copolymers (F127 and 10R5). Due to the fact that F127 shows low solubility in organic solvents, most studies involve the removal of dicyclohexylurea (side product) using ether solution, resulting in an unclean material for to medicinal applications. In our study, cleaner purification protocols were performed by using dialysis and size-exclusion column chromatography (SEC) in a water medium for obtaining F127-CF and 10R5-CF copolymers with in good yields (80–85%). Proton nuclear magnetic resonance (1H NMR) was employed to confirm the chemical structures of the final products.

中文翻译：

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作为潜在水溶性荧光探针的 5(6)-羧基荧光素共轭 F127 和 10R5 共聚物的合成和表征

摘要 在这项工作中，化学数据收集涉及基于 (5)6-羧基荧光素 (CF) 与正反三嵌段共聚物（F127 和 10R5）共轭的水溶性荧光探针的合成、纯化和表征。由于 F127 在有机溶剂中的溶解度低，大多数研究都涉及使用醚溶液去除二环己基脲（副产物），从而导致不干净的材料用于医疗应用。在我们的研究中，通过在水介质中使用透析和尺寸排阻柱色谱 (SEC) 来执行更清洁的纯化方案，以获得具有良好收率 (80-85%) 的 F127-CF 和 10R5-CF 共聚物。采用质子核磁共振 (1H NMR) 来确认最终产品的化学结构。