Histamine, the molecule associated with food poisoning, is nowadays considered as a reliable marker of seafood freshness and therefore its rapid and easy quantification appears critical. Existing method like HPLC or direct fluorimetric analysis employing ortho-phthalaldehyde (OPA) can be time-consuming and tedious. To overcome this problem, we propose in this paper to evaluate the application potential of 4-methoxy-ortho-phthalaldehyde (MO-OPA) for the rapid and easy evaluation of histamine in seafood samples. For this purpose, the histamine complexation conditions were studied by molecular fluorescence spectroscopy using MO-OPA as derivatization agent and then compared with the reference compounds: OPA. MOPA proved to be superior to OPA for histamine detection in terms of linearity range (1.10⁻⁶–1.10⁻⁴ mol.L⁻¹, R²>0.99, n = 10) and detection limit (9.5.10⁻⁷ mol.L⁻¹). The optimal conditions were next applied to the analysis of histamine in a mixture of biogenic amines standards and finally on real samples of yellowfin tuna of known histamine concentration.

组胺是与食物中毒有关的分子，如今被认为是海鲜新鲜度的可靠标志，因此其快速，简便的定量显得至关重要。现有的方法（例如HPLC或使用邻苯二甲醛（OPA）的直接荧光分析）可能既费时又乏味。为了克服这个问题，我们建议在本文中评估4-甲氧基邻苯二甲酸酯的应用潜力。-邻苯二甲醛（MO-OPA）用于快速轻松地评估海鲜样品中的组胺。为此，使用MO-OPA作为衍生剂，通过分子荧光光谱研究了组胺络合条件，然后与参考化合物：OPA进行了比较。MOPA证明是优于OPA组胺检测线性范围的术语（1.10 ^-6 -1.10 ^-4摩尔·升^-1，R 2> 0.99，N = 10）和检测限（9.5.10 ^-7 摩尔·升^{- 1}）。然后将最佳条件用于分析生物胺标准品混合物中的组胺，最后应用于已知组胺浓度的黄鳍金枪鱼的真实样品。