We report the fabrication of stable nanoclusters by salt-induced coupling of nanoparticles onto functionalized liposomes and their application in enhanced biosensing on a biomimetic surface. The coupling of nanoparticle-liposome structures and the subsequent debris removal were characterized by transmission electron microscopy (TEM) and absorption spectra. These nanoclusters were then tested for signal amplification with surface plasmon resonance (SPR), demonstrating advantages of an effective amplification by combining the large mass of lipid vesicles and the plasmonic properties of noble metal nanoparticles. Using 13 nm AuNPs-POPC nanoclusters, bacterial toxin detection was drastically enhanced with a markedly low nonspecific background, reaching a LOD of 0.1 ng/mL on a supported membrane interface. The nano-clusters prove to be a highly robust and reproducible signal amplification tag for optical biosensing, and also demonstrated a long shelf life, remain effective for over four weeks before showing signs of deterioration. A comparison between different types and sizes of noble metal nanoparticles with POPC liposomes was conducted and their effectiveness on amplification was assessed. The simple preparation procedure of liposome-nanoparticle nanoclusters reported here offers a promising bio-nanomaterial for sensitive biosensing and studies of vesicles-cell membrane interactions.

我们报告通过盐诱导耦合到功能化脂质体上的纳米粒子的耦合及其在增强的仿生生物表面上的生物传感中的应用来制造稳定的纳米团簇。纳米粒子-脂质体结构的耦合和随后的碎片清除通过透射电子显微镜（TEM）和吸收光谱进行了表征。然后测试了这些纳米簇的表面等离振子共振（SPR）信号放大，证明了通过结合大量脂质囊泡和贵金属纳米颗粒的等离子体性能有效放大的优势。使用13 nm AuNPs-POPC纳米簇，具有显着较低的非特异性背景，可大大增强细菌毒素检测，在支持的膜界面上的LOD达到0.1 ng / mL。纳米簇被证明是用于光学生物传感的高度健壮和可重现的信号放大标签，并且还显示出较长的保存期限，可以在显示出恶化迹象之前保持四周以上的有效性。进行了不同类型和大小的贵金属纳米颗粒与POPC脂质体之间的比较，并评估了它们对扩增的有效性。本文报道的脂质体-纳米颗粒纳米团簇的简单制备方法为敏感的生物传感和囊泡-细胞膜相互作用的研究提供了一种有希望的生物纳米材料。进行了不同类型和大小的贵金属纳米颗粒与POPC脂质体之间的比较，并评估了它们对扩增的有效性。本文报道的脂质体-纳米颗粒纳米团簇的简单制备方法为敏感的生物传感和囊泡-细胞膜相互作用的研究提供了一种有希望的生物纳米材料。进行了不同类型和大小的贵金属纳米颗粒与POPC脂质体之间的比较，并评估了它们对扩增的有效性。本文报道的脂质体-纳米颗粒纳米团簇的简单制备方法为敏感的生物传感和囊泡-细胞膜相互作用的研究提供了一种有希望的生物纳米材料。