The metabolism of Trichinella spp. is primarily anoxybiotic in nature. Their main energy source is glycogen, which is stored in the stichocites at the muscular stage of the larval development. When subject to tow temperatures the Trichinella larvae consume glycogen and neutral fats to provide for basal metabolism until the energy supplies reach the critical level. The present study establishes the glycogen concentration as well the invasive activity of T. nativа when affected by low temperatures in natural conditions. The carcasses of infected laboratory rats were placed in containers beneath the snow cover, in the natural conditions of a game husbandry in Central Russia. The viability, invasive capacity and the glycogen level were monitored in the Trichinella larvae monthly. The invasive capacity of Trichinella larvae was established based on the presence of the larvae in the muscular tissue of laboratory mice after the peroral administration of the helminth larvae. On the 45 day of the experiment, the mice were euthanized by cervical dislocation, and if the Trichinella larvae could be discovered in the muscular tissue with the help of the trichinelloscopic compression method, the invasive capacity of the Trichinella larvae was viewed as positive. To establish the quantitative value of glycogen content in Trichinella larvae a modified method was used. In order to measure the glycogen level in the T. nativa larvae isolated by fermentation larvae were counted in one drop of the suspended sedimentation in the Migacheva-Kotelnikov chamber. To establish the quantitative value of glycogen content in Trichinella larvae a method based on the treatment of glycogen with iodine, optical density measurement with a refractometer MКMФ-02 was used. For the purpose of measuring the concentration of glycogen in Trichinella larvae in the suspended sedimentation a calibration curve was used. The studies showed that the viability indicator of the Trichinella larvae which had been preserved in natural conditions in the four months of the winter-spring period, in the muscular tissue of laboratory rats remained high (over 90 %). The glycogen concentration in one helminth larva was 0.041 μg in January, 0.033 μg in February, 0.015 μg in April. The invasive capability of the preserved Trichinella larvae was considerably reduced to 33.3 %. In the winter period, under temperatures below 0 °C, a decrease in the glycogen concentration in the Trichinella larvae was observed.

旋毛虫属的代谢。本质上主要是缺氧的。它们的主要能量来源是糖原，在幼虫发育的肌肉阶段储存在粘滑石中。当处于两种温度下时，旋毛虫幼虫会消耗糖原和中性脂肪来提供基础代谢，直到能量供应达到临界水平。本研究确定了自然条件下低温影响下的糖原浓度以及T. nativа的入侵活性。受感染的实验室老鼠的尸体被放置在俄罗斯中部狩猎场的自然条件下，冰雪覆盖下的容器中。每月监测旋毛虫幼虫的活力、侵袭能力和糖原水平。旋毛虫幼虫的侵袭能力是根据口服蠕虫幼虫后实验室小鼠肌肉组织中存在的幼虫来确定的。实验第45天，颈椎脱位处死小鼠，若借助旋毛虫镜压迫法在肌肉组织中发现旋毛虫幼虫，则认为旋毛虫幼虫的侵袭能力为阳性。为了确定旋毛虫幼虫中糖原含量的定量值，使用了改进的方法。为了测量通过发酵分离的T. nativa幼虫中的糖原水平，对Migacheva-Kotelnikov室中悬浮沉降物的一滴幼虫进行计数。 为了确定旋毛虫幼虫糖原含量的定量值，采用基于用碘处理糖原的方法，使用折射计 MКMФ-02 进行光密度测量。为了测量悬浮沉降物中旋毛虫幼虫的糖原浓度，使用了校准曲线。研究表明，在冬春季节四个月的自然条件下保存的旋毛虫幼虫，在实验鼠肌肉组织中的存活率指标仍然很高（90%以上）。 1 月份蠕虫幼虫的糖原浓度为 0.041 μg，2 月份为 0.033 μg，4 月份为 0.015 μg。保存的旋毛虫幼虫的侵袭能力大大降低至33.3%。冬季，在低于0℃的温度下，观察到旋毛虫幼虫体内的糖原浓度下降。