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The role of PA-X C-terminal 20 residues of classical swine influenza virus in its replication and pathogenicity
Veterinary Microbiology ( IF 2.4 ) Pub Date : 2020-11-02 , DOI: 10.1016/j.vetmic.2020.108916
Xiu-Hui Wang 1 , Xiao-Qian Gong 2 , Feng Wen 3 , Bao-Yang Ruan 2 , Ling-Xue Yu 2 , Xiao-Min Liu 2 , Qi Wang 2 , Shuai-Yong Wang 2 , Juan Wang 2 , Yi-Feng Zhang 1 , Yan-Jun Zhou 2 , Tong-Ling Shan 2 , Wu Tong 2 , Hao Zheng 2 , Ning Kong 2 , Hai Yu 4 , Guang-Zhi Tong 5
Affiliation  

PA-X is a fusion protein encoded by a +1 frameshifted open reading frame (X-ORF) in PA gene. The X-ORF can be translated in full-length (61 amino acids, aa) or truncated (41 aa) form. However, the role of C-Terminal 20 aa of PA-X in virus function has not yet been fully elucidated. To this end, we constructed the contemporary influenza viruses with full and truncated PA-X by reverse genetics to compare their replication and pathogenicity. The full-length PA-X virus in MDCK and human A549 cells conferred 10- to 100-fold increase in viral replication, and more virulent and caused more severe inflammatory responses in mice relative to corresponding truncated PA-X virus, suggesting that the terminal 20 aa could play a role in enhancing viral replication and contribute to virulence.



中文翻译:

猪流感病毒PA-X C末端20个残基在其复制和致病性中的作用

PA-X是一种融合蛋白,由PA基因中的+1移码开放阅读框(X-ORF)编码。X-ORF可以全长(61个氨基酸,aa)或截短(41aa)的形式翻译。但是,PA-X的C末端20aa在病毒功能中的作用尚未完全阐明。为此,我们通过反向遗传学构建了具有完整和截短的PA-X的当代流感病毒,以比较它们的复制和致病性。与相应的截短的PA-X病毒相比,MDCK和人A549细胞中的全长PA-X病毒使病毒复制增加10至100倍,并且更具毒性,并引起更严重的炎症反应,提示小鼠20氨基酸可能在增强病毒复制和促进毒力中发挥作用。

更新日期:2020-11-13
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