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Maintaining Context in Ice: Cryo-EM/ET Workflow Optimizations
Structure ( IF 4.4 ) Pub Date : 2020-11-03 , DOI: 10.1016/j.str.2020.10.004
Nadav Elad , Sharon Grayer Wolf

In this issue of Structure, breakthroughs in cryo-EM/ET research are presented. Klebl et al. (2020) demonstrate how speed in sample vitrification impacts the quality of macromolecular particles in resultant cryo-EM grids. Wu et al. (2020) combine fluorescence, ion beam milling, and tomography to unravel unique features in vitrified yeast cells.



中文翻译:

在Ice中维护上下文:Cryo-EM / ET工作流程优化

在本期《结构》中,介绍了冷冻EM / ET研究的突破。Klebl等。(2020年)证明了样品玻璃化的速度如何影响所得冷冻EM网格中大分子颗粒的质量。Wu等。(2020)结合荧光,离子束铣削和层析成像技术来揭示玻璃化酵母细胞的独特特征。

更新日期:2020-11-03
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