Abstract Abdominal Aortic Aneurysm (AAA) is an enlargement with abdominal aorta, identified as the life-threatening complication, particularly with men at the age over 60. Circulating biomarkers are playing a role to diagnose AAA and matrix metalloproteinase-9 (MMP-9) was identified as the suitable biomarker for diagnosing conditions with AAA. Enzyme Linked Immunosorbent Assay (ELISA) is the standard technique to detect the target, further, a suitable surface modification on the polystyrene (PS) ELISA surface leads lowering the detection limit. Herein, a potential bimolecular immobilization strategy was introduced by complexing silane-antibody or silane-gold-antibody for the efficient determination of MMP-9 detection. Further, sandwich pattern was followed to determine MMP-9 by poly- and mono-clonal antibodies and compared. Detection limit was found as 1 pM, which is much lower than conventional ELISA (1.5 nM) and 10 pM on silane-modified PS surface. Moreover, MMP-9 was also detected in human serum at 1:35 dilution, helps to quantify the level of MMP-9 for diagnosing AAA and its associated condition.

中文翻译：

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金-硅烷复合抗体固定在聚苯乙烯 ELISA 表面，用于增强测定基质金属蛋白酶-9

摘要 腹主动脉瘤 (AAA) 是腹主动脉肿大，被确定为危及生命的并发症，尤其是 60 岁以上的男性。循环生物标志物在诊断 AAA 和基质金属蛋白酶 9 (MMP-9) 中发挥作用被确定为诊断 AAA 疾病的合适生物标志物。酶联免疫吸附测定 (ELISA) 是检测靶标的标准技术，此外，聚苯乙烯 (PS) ELISA 表面上的适当表面修饰可降低检测限。在此，通过络合硅烷-抗体或硅烷-金-抗体引入了一种潜在的双分子固定化策略，用于有效测定 MMP-9 检测。此外，遵循夹心模式通过多克隆和单克隆抗体确定 MMP-9 并进行比较。发现检测限为 1 pM，远低于传统 ELISA (1.5 nM) 和硅烷改性 PS 表面的 10 pM。此外，还在 1:35 稀释的人血清中检测到 MMP-9，有助于量化 MMP-9 的水平，用于诊断 AAA 及其相关疾病。