Alginate–gelatin (Alg–Gel) composite hydrogel is extensively used in extrusion-based bioprinting. Although Alg–Gel blends possess excellent biocompatibility and printability, poor mechanical properties have hindered its further clinical applications. In this study, a series of design by incorporating bioactive glass nanoparticles (BG) (particle size of 12 and 25 nm) into Alg–Gel hydrogel have been considered for optimizing the mechanical and biological properties. The composite Alg–Gel–BG bioink was biophysically characterized by mechanical tests and bioprinting practice. Biocompatibility of Alg–Gel–BG bioink was then investigated by bioprinting mouse dermal fibroblasts. Mechanical tests showed enhanced stiffness with increasing concentration of incorporated BG. But the maximum concentration of BG was determined 1.0 wt% before blends became too viscous to print. Meanwhile, the incorporation of BG did not affect the highly porous structure and biodegradation of Alg–Gel hydrogel, while the mechanical strength and printability were enhanced. In addition, the cellular proliferation and adhesion in the bioprinted constructs were significantly enhanced by BG (12 nm), while extension was not affected. Therefore, our strategy of incorporating BG in Alg–Gel composite hydrogel represents an easy-to-use approach to the mechanical reinforcement of cell-laden bioink, thus demonstrating their suitability for future applications in extrusion-based bioprinting.

藻酸盐-明胶（Alg-Gel）复合水凝胶广泛用于基于挤出的生物打印。尽管 Alg-Gel 混合物具有优异的生物相容性和可印刷性，但较差的机械性能阻碍了其进一步的临床应用。在这项研究中，通过将生物活性玻璃纳米粒子 (BG)（粒径为 12 和 25 nm）结合到 Alg-Gel 水凝胶中的一系列设计已被考虑用于优化机械和生物性能。复合 Alg-Gel-BG 生物墨水通过机械测试和生物打印实践进行生物物理表征。然后通过生物打印小鼠真皮成纤维细胞来研究 Alg-Gel-BG bioink 的生物相容性。机械测试表明，随着掺入的 BG 浓度的增加，刚度增强。但BG的最大浓度确定为1。在混合物变得太粘而无法打印之前为 0 wt%。同时，BG 的掺入不影响 Alg-Gel 水凝胶的高孔隙结构和生物降解性，同时增强了机械强度和可印刷性。此外，BG（12 nm）显着增强了生物打印结构中的细胞增殖和粘附，而延伸不受影响。因此，我们在 Alg-Gel 复合水凝胶中加入 BG 的策略代表了一种易于使用的方法来机械增强载有细胞的生物墨水，从而证明它们适用于未来在基于挤出的生物打印中的应用。BG (12 nm) 显着增强了生物打印结构中的细胞增殖和粘附，而延伸不受影响。因此，我们在 Alg-Gel 复合水凝胶中加入 BG 的策略代表了一种易于使用的方法来机械增强载有细胞的生物墨水，从而证明它们适用于未来在基于挤出的生物打印中的应用。BG (12 nm) 显着增强了生物打印结构中的细胞增殖和粘附，而延伸不受影响。因此，我们在 Alg-Gel 复合水凝胶中加入 BG 的策略代表了一种易于使用的方法来机械增强载有细胞的生物墨水，从而证明它们适用于未来在基于挤出的生物打印中的应用。