Abstract

Biological nanoparticles are considered to improve treatment with the least negative complications. Selenium nanoparticles have been used to treat diseases such as pathogenic infections and cancers. In this study, the effect of selenium nanoparticles on macrophage responses was evaluated. Selenium nanoparticles were synthesized biologically by Lactobacillus Plantarum and confirmed through TEM and SEM microscopy, EDX, and XRD characterizations. Macrophage cells were cultured and treated only with selenium nanoparticles and in combination with the bladder tumor lysate. Moreover, tumor lysate only was used as the other group. The genes expression of IFN-γ, IL-27, IL-12, and MHC-I was assessed by real-time PCR method. The production levels of IFN-γ and IL-27 cytokines were evaluated by ELISA assay. Selenium nanoparticles in combination with the tumor lysate increased the genes expression of IFN-γ, IL-27, IL-12, and MHC-I after 24 h and 48 h of treatment. Also, IFN-γ and IL-27 production was increased in the group treated with selenium nanoparticles in combination with the tumor lysate after 48 h and 72 h of treatment. The highest effect of selenium nanoparticles was observed after 48 and 72 h of treatment in the genes expression and cytokines production, respectively. Therefore, the optimum effect of selenium nanoparticle acts as a treatment dependent-manner.

中文翻译：

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生物硒纳米粒子修饰暴露于膀胱肿瘤抗原的巨噬细胞的免疫反应。

摘要

生物纳米颗粒被认为可以改善治疗，同时减少不良并发症。硒纳米颗粒已用于治疗疾病，例如病原体感染和癌症。在这项研究中，评估了硒纳米颗粒对巨噬细胞反应的影响。植物乳杆菌生物合成硒纳米颗粒并通过TEM和SEM显微镜，EDX和XRD表征得到证实。培养巨噬细胞并仅用硒纳米颗粒并与膀胱肿瘤溶解产物组合处理。此外，仅将肿瘤裂解物用作另一组。通过实时PCR方法评估IFN-γ，IL-27，IL-12和MHC-1的基因表达。通过ELISA测定评估IFN-γ和IL-27细胞因子的产生水平。硒纳米颗粒与肿瘤溶解产物的结合在治疗24小时和48小时后增加了IFN-γ，IL-27，IL-12和MHC-1的基因表达。同样，在治疗48小时和72小时后，用硒纳米颗粒与肿瘤溶解产物联合治疗的组中IFN-γ和IL-27的产生增加。在处理48和72小时后，分别观察到硒纳米颗粒的基因表达和细胞因子产生效果最高。因此，硒纳米颗粒的最佳效果是依赖治疗的方式。