Cardamom (Elettaria cardamomum Maton) is a highly valuable commercial spice crop, originating from the Western Ghats of southern India. It has importance both as a spice and for its medicinal properties. An efficient and reliable micropropagation protocol using shoot tips as explants is presented which can be utilized for development of elite planting material. Growth patterns of cardamom shoots were analyzed on 45 media treatments and best shoot proliferation was recorded on Murashige and Skoog (MS) medium supplemented with 4.4 μM 6-benzylaminopurine (BAP) and 2.32 μM kinetin (Kn) with 5.83 shoots regenerated per explant. The longest shoots (6 cm) were observed on 0.44 μM BAP and 2.32 μM Kn containing medium. Regenerated shoots rooted well on full strength MS basal medium, generating 3.50 roots per explant with an average root length of 4.33 cm within 4 wk. Successful hardening with a field survival percentage of 80% was achieved after acclimatization of well-developed plantlets. Genetic stability of the micropropagated plants was confirmed using 13 ISSR markers. This study reports an improved micropropagation protocol for cardamom with an enhanced rate of shoot multiplication leading to the production of healthy shoots which can be easily rooted and successfully transferred to the field.

豆蔻（Elettaria豆蔻Maton）是一种非常有价值的商业香料作物，起源于印度南部的西高止山脉。它作为香料及其药用特性都具有重要意义。提出了一种有效且可靠的微繁殖方案，该方法以芽尖为外植体，可用于开发优良的种植材料。在45种培养基处理中分析了豆蔻幼苗的生长模式，并在补充了4.4μM6-苄基氨基嘌呤（BAP）和2.32μM激动素（Kn）的Murashige和Skoog（MS）培养基上记录了最佳的芽增殖，每个外植体再生了5.83芽。在含0.44μMBAP和2.32μMKn的培养基上观察到最长的芽（6厘米）。再生的枝条在全强度MS基础培养基上很好地生根，每个植株产生3.50根，在4周内平均根长为4.33 cm。在使成熟的小苗适应环境之后，成功地实现了80％的野外存活率。使用13个ISSR标记确认了微繁殖植物的遗传稳定性。这项研究报告了一种改进的豆蔻微繁殖方案，增加了芽的繁殖率，从而导致了可以轻松生根并成功转移到田间的健康芽的生产。