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Deterministic droplet coding via acoustofluidics
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-10-26 , DOI: 10.1039/d0lc00538j Peiran Zhang 1 , Wei Wang , Hai Fu , Joseph Rich , Xingyu Su , Hunter Bachman , Jianping Xia , Jinxin Zhang , Shuaiguo Zhao , Jia Zhou , Tony Jun Huang
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-10-26 , DOI: 10.1039/d0lc00538j Peiran Zhang 1 , Wei Wang , Hai Fu , Joseph Rich , Xingyu Su , Hunter Bachman , Jianping Xia , Jinxin Zhang , Shuaiguo Zhao , Jia Zhou , Tony Jun Huang
Affiliation
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Droplet microfluidics has become an indispensable tool for biomedical research and lab-on-a-chip applications owing to its unprecedented throughput, precision, and cost-effectiveness. Although droplets can be generated and screened in a high-throughput manner, the inability to label the inordinate amounts of droplets hinders identifying the individual droplets after generation. Herein, we demonstrate an acoustofluidic platform that enables on-demand, real-time dispensing, and deterministic coding of droplets based on their volumes. By dynamically splitting the aqueous flow using an oil jet triggered by focused traveling surface acoustic waves, a sequence of droplets with deterministic volumes can be continuously dispensed at a throughput of 100 Hz. These sequences encode barcoding information through the combination of various droplet lengths. As a proof-of-concept, we encoded droplet sequences into end-to-end packages (e.g., a series of 50 droplets), which consisted of an address barcode with binary volumetric combinations and a sample package with consistent volumes for hosting analytes. This acoustofluidics-based, deterministic droplet coding technique enables the tagging of droplets with high capacity and high error-tolerance, and can potentially benefit various applications involving single cell phenotyping and multiplexed screening.
中文翻译：
通过声流控确定性液滴编码
液滴微流控因其前所未有的通量、精度和成本效益，已成为生物医学研究和芯片实验室应用不可或缺的工具。尽管可以以高通量方式生成和筛选液滴，但无法标记过量的液滴阻碍了生成后识别单个液滴。在这里，我们演示了一个声流控平台，可以根据液滴的体积进行按需、实时分配和确定性编码。通过使用聚焦行进表面声波触发的油喷射动态分裂水流，可以以 100 Hz 的吞吐量连续分配一系列具有确定体积的液滴。这些序列通过不同液滴长度的组合来编码条形码信息。作为概念验证，我们将液滴序列编码为端到端包（例如，一系列 50 个液滴），其中包含具有二进制体积组合的地址条形码和具有用于托管分析物的一致体积的样品包。这种基于声流控的确定性液滴编码技术能够以高容量和高容错性标记液滴，并且可能有利于涉及单细胞表型分析和多重筛选的各种应用。
更新日期：2020-11-03
中文翻译：
通过声流控确定性液滴编码
液滴微流控因其前所未有的通量、精度和成本效益，已成为生物医学研究和芯片实验室应用不可或缺的工具。尽管可以以高通量方式生成和筛选液滴，但无法标记过量的液滴阻碍了生成后识别单个液滴。在这里，我们演示了一个声流控平台，可以根据液滴的体积进行按需、实时分配和确定性编码。通过使用聚焦行进表面声波触发的油喷射动态分裂水流，可以以 100 Hz 的吞吐量连续分配一系列具有确定体积的液滴。这些序列通过不同液滴长度的组合来编码条形码信息。作为概念验证，我们将液滴序列编码为端到端包（例如，一系列 50 个液滴），其中包含具有二进制体积组合的地址条形码和具有用于托管分析物的一致体积的样品包。这种基于声流控的确定性液滴编码技术能够以高容量和高容错性标记液滴，并且可能有利于涉及单细胞表型分析和多重筛选的各种应用。
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