To investigate RNA degradation in live cells, detection methods that do not require RNA extraction from cells are necessary. In this study, we examined the utility of fluorescence lifetime measurements using a probe attached to the end of an RNA molecule for detecting RNA degradation. We optimized a short fluorescein-labeled RNA sequence whose fluorescence lifetime varied significantly before and after degradation. The selected HHG-fluorescein sequence (H = U, C, or A) is a promising RNA labeling unit (fluorescence lifetime probe) for live cell imaging of RNA degradation.

中文翻译：

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用于检测RNA降解的荧光寿命探针

为了研究活细胞中的RNA降解，不需要从细胞中提取RNA的检测方法是必要的。在这项研究中，我们使用连接到RNA分子末端的探针检测RNA降解，检验了荧光寿命测量的实用性。我们优化了短的荧光素标记的RNA序列，其降解前后的荧光寿命显着不同。所选的HHG-荧光素序列（H = U，C或A）是用于RNA降解的活细胞成像的有前途的RNA标记单位（荧光寿命探针）。