A spectrophotometric method has been studied for the determination of epsilon aminocaproic acid (EACA) through charge transfer (CT) complexation with bromanil (TBBQ). The CT reaction has been carried out at 70 °C of the water bath for 30 min in the borax buffer solution. The spectrum obtained for EACA/TBBQ system shows the maximum absorption band at wavelength of 352 nm. The CT complex is also confirmed by both FTIR and ¹H NMR measurements. The stoichiometry of the CT complex is found to be 1:1 ratio by Job and straight line methods between the donor and the acceptor. At the optimum reaction conditions, Beer’s law is obeyed in a concentration limit of 2～8 µg mL⁻¹. The relative standard deviation is less than 1.5%. The apparent molar absorptivity is determined to be 1.16×10⁴ L mol⁻¹ cm⁻¹ at 352 nm. The thermodynamic properties and reaction mechanism of the CT complexation have been discussed. The developed method could be applied successfully for the determination of the studied compound in its pharmaceutical dosage form with a good precision and accuracy compared to official method as revealed by t- and F-tests.

中文翻译：

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ε-氨基己酸与溴的电荷转移络合的分光光度特性研究

已经研究了一种分光光度法，通过与溴腈（TBBQ）的电荷转移（CT）络合测定ε-氨基己酸（EACA）。CT反应已在硼砂缓冲液中的70°C水浴中进行了30分钟。EACA / TBBQ系统获得的光谱显示出在352 nm波长处的最大吸收带。CT复合物也通过FTIR和¹ H NMR测定得到证实。通过Job法和直线法在供体和受体之间发现CT复合物的化学计量比为1：1。在最佳反应条件下，遵守比尔定律的浓度极限为2〜8 µg mL ^{− 1}。相对标准偏差小于1.5％。表观摩尔吸光被确定为1.16×10 ⁴大号摩尔^{- 1}厘米^{- 1}在352纳米。讨论了CT络合的热力学性质和反应机理。与t-和F-测试所示的官方方法相比，该方法可以成功地用于确定药物剂型中所研究化合物的精密度和准确性。