The distribution of β-lactam resistance genes in P. aeruginosa is often closely related to the distribution of certain high-risk international clones. We used whole-genome sequencing (WGS) to identify the predominant sequence types (ST) and β-lactamase genes in clinical isolates of multidrug-resistant (MDR)-P. aeruginosa from Qatar Microbiological identification and susceptibility tests were performed by automated BD Phoenix™ system and manual Liofilchem MIC Test Strips. Among 75 MDR-P. aeruginosa isolates; the largest proportions of susceptibility were to ceftazidime-avibactam (n = 36, 48%), followed by ceftolozane-tazobactam (30, 40%), ceftazidime (n = 21, 28%) and aztreonam (n = 16, 21.3%). All isolates possessed Class C and/or Class D β-lactamases (n = 72, 96% each), while metallo-β-lactamases were detected in 20 (26.7%) isolates. Eight (40%) metallo-β-lactamase producers were susceptible to aztreonam and did not produce any concomitant extended-spectrum β-lactamases. High risk ST235 (n = 16, 21.3%), ST357 (n = 8, 10.7%), ST389 and ST1284 (6, 8% each) were most frequent. Nearly all ST235 isolates (15/16; 93.8%) were resistant to all tested β-lactams. MDR-P. aeruginosa isolates from Qatar are highly resistant to antipseudomonal β-lactams. High-risk STs are predominant in Qatar and their associated MDR phenotypes are a cause for considerable concern.

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来自卡塔尔的 MDR-铜绿假单胞菌 β-内酰胺酶介导的耐药性

铜绿假单胞菌中β-内酰胺抗性基因的分布往往与某些国际高危克隆的分布密切相关。我们使用全基因组测序 (WGS) 来鉴定多药耐药 (MDR)-P 临床分离株中的主要序列类型 (ST) 和 β-内酰胺酶基因。来自卡塔尔的铜绿假单胞菌微生物鉴定和敏感性测试通过自动 BD Phoenix™ 系统和手动 Liofilchem MIC 测试条进行。其中 75 MDR-P。铜绿假单胞菌; 最大比例的易感性是头孢他啶-阿维巴坦 (n = 36, 48%)，其次是头孢他啶-他唑巴坦 (30, 40%)、头孢他啶 (n = 21, 28%) 和氨曲南 (n = 16, 21.3%) . 所有分离株都具有 C 类和/或 D 类 β-内酰胺酶（n = 72，各占 96%），而在 20 个（26.7%）分离株中检测到金属-β-内酰胺酶。八个 (40%) 金属-β-内酰胺酶生产者对氨曲南敏感，并且不产生任何伴随的超广谱 β-内酰胺酶。高风险 ST235 (n = 16, 21.3%)、ST357 (n = 8, 10.7%)、ST389 和 ST1284 (6, 8%) 最常见。几乎所有 ST235 分离株（15/16；93.8%）对所有测试的 β-内酰胺类药物都具有抗性。MDR-P。来自卡塔尔的铜绿假单胞菌对抗假单胞菌β-内酰胺类具有高度耐药性。高风险 ST 在卡塔尔占主导地位，其相关的 MDR 表型引起了相当大的关注。来自卡塔尔的铜绿假单胞菌对抗假单胞菌β-内酰胺类具有高度耐药性。高风险 ST 在卡塔尔占主导地位，其相关的 MDR 表型引起了相当大的关注。来自卡塔尔的铜绿假单胞菌对抗假单胞菌β-内酰胺类具有高度耐药性。高风险 ST 在卡塔尔占主导地位，其相关的 MDR 表型引起了相当大的关注。