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Aqueous Suspensions of Cellulose Oligomer Nanoribbons for Growth and Natural Filtration-Based Separation of Cancer Spheroids
Langmuir ( IF 3.7 ) Pub Date : 2020-11-02 , DOI: 10.1021/acs.langmuir.0c02294 Takeshi Serizawa 1 , Tohru Maeda 1 , Saeko Yamaguchi 1 , Toshiki Sawada 1, 2
Langmuir ( IF 3.7 ) Pub Date : 2020-11-02 , DOI: 10.1021/acs.langmuir.0c02294 Takeshi Serizawa 1 , Tohru Maeda 1 , Saeko Yamaguchi 1 , Toshiki Sawada 1, 2
Affiliation
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In vitro growth of cancer spheroids (CSs) and the subsequent separation of CSs from a 2D or 3D cell culture system are important for fundamental cancer studies and cancer drug screening. Although biopolymer-based or synthetic hydrogels are suitable candidates to be used as 3D cell culture scaffolds, alternatives with better processing capabilities are still required to set up cell culture microenvironment. In this study, we show that aqueous suspensions of crystalline nanoribbons composed of cellulose oligomers have a potential for CS growth and separation. The nanoribbon suspensions in serum-containing cell culture media fixed single cancer cells and CSs with large sizes in a 3D space, leading to suspension cultures for CS growth corresponding to culture time. Well-grown CSs were easily separated from the suspensions by natural filtration using a mesh filter with a suitable pore size. Cell viability tests revealed negligible cytotoxicity of the nanoribbons. In addition, physical damages to CSs by the separation procedures were negligible. Stable suspensions of biocompatible nanomaterials will thus provide novel microenvironments for growth and separation of diverse cell aggregates.
中文翻译：
纤维素低聚物纳米带的水性悬浮液用于癌症球体的生长和基于自然过滤的分离
癌症球体（CSs）的体外生长以及随后将CSs从2D或3D细胞培养系统中分离出来对于基础癌症研究和癌症药物筛选至关重要。尽管基于生物聚合物的或合成的水凝胶是适合用作3D细胞培养支架的候选人，但仍需要具有更好处理能力的替代品来建立细胞培养微环境。在这项研究中，我们表明由纤维素低聚物组成的结晶纳米带的水悬浮液具有CS生长和分离的潜力。含有血清的细胞培养基中的纳米带悬浮液将单个癌细胞和CS固定在3D空间中，具有较大的尺寸，从而导致悬浮培养物的CS生长对应于培养时间。通过使用具有合适孔径的筛网过滤器进行自然过滤，可以轻松地将生长良好的CS从悬浮液中分离出来。细胞活力测试表明，纳米带的细胞毒性可忽略不计。此外，分离程序对CS的物理损害可以忽略不计。因此，生物相容性纳米材料的稳定悬浮液将为多种细胞聚集体的生长和分离提供新型的微环境。
更新日期：2020-11-25
中文翻译：
纤维素低聚物纳米带的水性悬浮液用于癌症球体的生长和基于自然过滤的分离
癌症球体（CSs）的体外生长以及随后将CSs从2D或3D细胞培养系统中分离出来对于基础癌症研究和癌症药物筛选至关重要。尽管基于生物聚合物的或合成的水凝胶是适合用作3D细胞培养支架的候选人，但仍需要具有更好处理能力的替代品来建立细胞培养微环境。在这项研究中，我们表明由纤维素低聚物组成的结晶纳米带的水悬浮液具有CS生长和分离的潜力。含有血清的细胞培养基中的纳米带悬浮液将单个癌细胞和CS固定在3D空间中，具有较大的尺寸，从而导致悬浮培养物的CS生长对应于培养时间。通过使用具有合适孔径的筛网过滤器进行自然过滤，可以轻松地将生长良好的CS从悬浮液中分离出来。细胞活力测试表明，纳米带的细胞毒性可忽略不计。此外，分离程序对CS的物理损害可以忽略不计。因此，生物相容性纳米材料的稳定悬浮液将为多种细胞聚集体的生长和分离提供新型的微环境。
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