In many invertebrates with external fertilization, synchrony spawning is an important behavior to increase fertilization success. The past physiological study demonstrated that the highly reactive species could induce spawning behavior in the tank experiments, however, the stimulus to induce spawning have not been clear in the wild. Our study species Ezo abalone Haliotis discus hannai mainly spawn during a stormy weather. Rainwater contain H₂O₂ and iron (II) ions (Fe²⁺). The stormy weather could mix layer of water, and the water of surface layer containing H₂O₂ and Fe²⁺ could reach the sea bottom. Hydroxyl radical (^・OH), which is a kind of the highly reactive species, could be generated in the process of H₂O₂ oxidizing Fe²⁺ (Fenton reaction). Therefore, we hypothesized that Fenton reaction occurs on the sea bottom during a stormy condition, and ^・OH generated by Fenton reaction induce the spawning behavior of abalone in the wild. This study observed that H. discus hannai released eggs after salinity decreased due to the rainfall during the stormy conditions. In addition, our tank experiment demonstrated that ･OH generated by Fenton reaction induce the synchronous spawning behavior between the sexes. This study provides a new hypothesis about the spawning process in H. discus hannai and which the hypothesis could explain that of other invertebrates.

中文翻译：

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在暴风雨天气下，可能的刺激物导致埃佐鲍鱼鲍氏鲍氏鲍氏菌出现裂口：芬顿反应引起产卵行为

在许多外部受精的无脊椎动物中，同步产卵是增加受精成功率的重要行为。过去的生理研究表明，高反应性物种可以在坦克实验中诱导产卵行为，但是，在野外尚不清楚刺激产卵的刺激因素。我们的研究物种Ezo鲍鱼Haliotis discus hannai主要在暴风雨天气产卵。雨水包含H ₂ O ₂和铁（II）离子（Fe ²⁺）。暴风雨天气可能会混入水层，而含有H ₂ O ₂和Fe ²⁺的表层水可能会到达海底。羟自由基（^・H ₂ O ₂氧化Fe ²⁺（Fenton反应）的过程中可能会生成一种高反应性的OH ）。因此，我们假设在暴风雨期间海底会发生Fenton反应，并且Fenton反应产生的^· OH会诱导野生鲍鱼的产卵行为。这项研究发现，由于暴风雨期间的降雨，盐度降低后，H.discus hannai释放了卵。此外，我们的坦克实验表明，芬顿反应产生的OH引起两性之间的同步产卵行为。这项研究提供了一个关于H.discus hannai产卵过程的新假设。 假说可以解释其他无脊椎动物的假说。