当前位置：
X-MOL 学术
›
Biomacromolecules
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Enhanced Osteogenic Potential of Phosphonated-Siloxane Hydrogel Scaffolds
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-11-02 , DOI: 10.1021/acs.biomac.0c01293 Michael T Frassica 1 , Sarah K Jones 1 , Jakkrit Suriboot 1 , Ahmad S Arabiyat 2 , Esteban M Ramirez 1 , Robert A Culibrk 2 , Mariah S Hahn 2 , Melissa A Grunlan 1, 3, 4
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-11-02 , DOI: 10.1021/acs.biomac.0c01293 Michael T Frassica 1 , Sarah K Jones 1 , Jakkrit Suriboot 1 , Ahmad S Arabiyat 2 , Esteban M Ramirez 1 , Robert A Culibrk 2 , Mariah S Hahn 2 , Melissa A Grunlan 1, 3, 4
Affiliation
|
In a material-guided approach, instructive scaffolds that leverage potent chemistries may efficiently promote bone regeneration. A siloxane macromer has been previously shown to impart osteoinductivity and bioactivity when included in poly(ethylene glycol) diacrylate (PEG-DA) hydrogel scaffolds. Herein, phosphonated-siloxane macromers were evaluated for enhancing the osteogenic potential of siloxane-containing PEG-DA scaffolds. Two macromers were prepared with different phosphonate pendant group concentrations, poly(diethyl(2-(propylthio)ethyl)phosphonate methylsiloxane) diacrylate (PPMS-DA) and 25%-phosphonated analogue (PPMS-DA 25%). Macroporous, templated scaffolds were prepared by cross-linking these macromers with PEG-DA at varying mol % (15:85, 30:70, and 45:55 PPMS-DA to PEG-DA; 30:70 PPMS-DA 25% to PEG-DA). Other scaffolds were also prepared by combining PEG-DA with PDMS-MA (i.e., no phosphonate) or with vinyl phosphonate (i.e., no siloxane). Scaffold material properties were thoroughly assessed, including pore morphology, hydrophobicity, swelling, modulus, and bioactivity. Scaffolds were cultured with human bone marrow-derived mesenchymal stem cells (normal media) and calcium deposition and protein expression were assessed at 14 and 28 days.
中文翻译：
膦酸硅氧烷水凝胶支架的成骨潜能增强。
在材料指导的方法中，利用有效化学作用的指导性支架可以有效地促进骨再生。先前已证明，硅氧烷大分子单体包含在聚（乙二醇）二丙烯酸酯（PEG-DA）水凝胶支架中时具有骨诱导性和生物活性。在本文中，评估了膦酸酯化的硅氧烷大分子单体以增强含硅氧烷的PEG-DA支架的成骨潜力。制备具有不同膦酸酯侧基浓度的两个大分子单体，聚（二乙基（2-（丙硫基乙基）膦酸酯甲基硅氧烷）二丙烯酸酯（PPMS-DA）和25％的膦酸酯类似物（PPMS-DA 25％）。通过将这些大分子单体与PEG-DA以不同摩尔％（15：85、30：70和45:55 PPMS-DA与PEG-DA; 30:70 PPMS-DA 25％至PEG-DA）。还通过将PEG-DA与PDMS-MA（即，没有膦酸酯）或与乙烯基膦酸酯（即，没有硅氧烷）结合来制备其他支架。对支架材料的性能进行了全面评估，包括孔的形态，疏水性，溶胀，模量和生物活性。将支架与人骨髓源间充质干细胞（正常培养基）一起培养，并在第14天和第28天评估钙的沉积和蛋白质表达。
更新日期：2020-12-14
中文翻译：
膦酸硅氧烷水凝胶支架的成骨潜能增强。
在材料指导的方法中，利用有效化学作用的指导性支架可以有效地促进骨再生。先前已证明，硅氧烷大分子单体包含在聚（乙二醇）二丙烯酸酯（PEG-DA）水凝胶支架中时具有骨诱导性和生物活性。在本文中，评估了膦酸酯化的硅氧烷大分子单体以增强含硅氧烷的PEG-DA支架的成骨潜力。制备具有不同膦酸酯侧基浓度的两个大分子单体，聚（二乙基（2-（丙硫基乙基）膦酸酯甲基硅氧烷）二丙烯酸酯（PPMS-DA）和25％的膦酸酯类似物（PPMS-DA 25％）。通过将这些大分子单体与PEG-DA以不同摩尔％（15：85、30：70和45:55 PPMS-DA与PEG-DA; 30:70 PPMS-DA 25％至PEG-DA）。还通过将PEG-DA与PDMS-MA（即，没有膦酸酯）或与乙烯基膦酸酯（即，没有硅氧烷）结合来制备其他支架。对支架材料的性能进行了全面评估，包括孔的形态，疏水性，溶胀，模量和生物活性。将支架与人骨髓源间充质干细胞（正常培养基）一起培养，并在第14天和第28天评估钙的沉积和蛋白质表达。
京公网安备 11010802027423号