There has been much recent interest in the protein “corona,” the nonspecific adsorption of proteins on the surface of nanoparticles used in biological applications. This research investigates an analogous DNA corona. We find that particles (200 nm and 1 μm) incubated with DNA form a DNA corona, with a higher concentration of DNA adsorbed on the surface of cationic nanoparticles. With protein present, a combined DNA and protein corona is formed although DNA in solution displaces protein from the nanoparticle surface. Displacement of protein from the nanoparticle surface is dependent on the concentration of DNA in solution and was also observed for planar surfaces. Overall, we expect this investigation of the DNA corona to be important for nanomedicine applications, as well as disease states, especially systemic lupus erythematosus, in which biological particles with bound DNA are important mediators of inflammation and thrombosis.

中文翻译：

---

DNA-纳米粒子相互作用：DNA 冠的形成及其对蛋白质冠的影响


最近人们对蛋白质“电晕”产生了很大的兴趣，即蛋白质在生物应用中使用的纳米颗粒表面的非特异性吸附。这项研究调查了类似的 DNA 电晕。我们发现颗粒（200 nm 和 1 μm ）与 DNA 一起孵育形成 DNA 冠，阳离子纳米颗粒表面吸附有较高浓度的 DNA。尽管溶液中的 DNA 取代了纳米颗粒表面的蛋白质，但随着蛋白质的存在，形成了组合的 DNA 和蛋白质冠。蛋白质从纳米颗粒表面的置换取决于溶液中 DNA 的浓度，并且在平面表面也观察到了这种情况。总体而言，我们预计 DNA 电晕的这项研究对于纳米医学应用以及疾病状态非常重要，特别是系统性红斑狼疮，其中结合 DNA 的生物颗粒是炎症和血栓形成的重要介质。