Combination of various compounds and essential oils for pharmaceutical formulations withdraw attention. In this present study, it was aimed to evaluate the in vitro potential synergistic antibacterial effect of Lavandula latifolia (spike lavender) essential oil with camphor by using the checkerboard method against the human pathogens; Staphylococcus aureus and Listeria monocytogenes. Pharmacopoeia quality L. latifolia essential oil and racemic camphor were analyzed and verified by GC-FID and GC/MS, simultaneously. In vitro antibacterial activity of essential oil and camphor (MIC range: 0.16-20 mg/mL) and standard antimicrobial clarithromycin (MIC range: 0.125-16 μg/mL) were carried out by broth microdilution against S. aureus and L. monocytogenes standard strains, respectively. Resulting antibacterial effects were evaluated for their fractional inhibitory concentrations (FICs) as antagonistic, additive and synergistic effects. The analytical results showed that the major component of essential oil was linalool (45.2%) and 1,8-cineole (25.6%). Antibacterial effects of essential oil were determined as MIC 1.25-5 mg/mL. As a result of the experiments, L. latifolia essential oil-camphor combinations were identified as "synergistic (FIC ≤ 0.5), and additive (0.5 < FIC ≤ 1)" in the respective combinations, suggesting further evaluation for formulations for potential antimicrobial applications in food and pharmaceuticals.

中文翻译：

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Lavandula latifolia Medik 的协同抗菌组合。香樟精油

用于药物制剂的各种化合物和精油的组合引起了人们的注意。在本研究中，旨在通过使用棋盘法对抗人类病原体，评估 Lavandula latifolia（穗薰衣草）精油与樟脑的体外潜在协同抗菌作用；金黄色葡萄球菌和单核细胞增生李斯特菌。通过GC-FID和GC/MS同时分析和验证药典质量的阔叶松精油和外消旋樟脑。精油和樟脑（MIC 范围：0.16-20 mg/mL）和标准抗菌克拉霉素（MIC 范围：0.125-16 μg/mL）的体外抗菌活性通过针对金黄色葡萄球菌和单核细胞增生李斯特菌标准的肉汤微量稀释进行株，分别。由此产生的抗菌作用被评估为它们的部分抑制浓度 (FIC) 作为拮抗、加和和协同作用。分析结果表明，精油的主要成分是芳樟醇（45.2%）和1,8-桉树脑（25.6%）。精油的抗菌作用被确定为 MIC 1.25-5 mg/mL。实验结果表明，阔叶树精油-樟脑组合在各自的组合中具有“协同作用（FIC ≤ 0.5）和添加剂（0.5 < FIC ≤ 1）”，这表明对潜在抗菌应用的配方进行进一步评估在食品和药品方面。6%）。精油的抗菌作用被确定为 MIC 1.25-5 mg/mL。实验结果表明，阔叶树精油-樟脑组合在各自的组合中具有“协同作用（FIC ≤ 0.5）和添加剂（0.5 < FIC ≤ 1）”，这表明对潜在抗菌应用的配方进行进一步评估在食品和药品方面。6%）。精油的抗菌作用被确定为 MIC 1.25-5 mg/mL。实验结果表明，阔叶树精油-樟脑组合在各自的组合中具有“协同作用（FIC ≤ 0.5）和添加剂（0.5 < FIC ≤ 1）”，这表明对潜在抗菌应用的配方进行进一步评估在食品和药品方面。