Achieving a comprehensive understanding of brain function requires multiple imaging modalities with complementary strengths. We present an approach for concurrent widefield optical and functional magnetic resonance imaging. By merging these modalities, we can simultaneously acquire whole-brain blood-oxygen-level-dependent (BOLD) and whole-cortex calcium-sensitive fluorescent measures of brain activity. In a transgenic murine model, we show that calcium predicts the BOLD signal, using a model that optimizes a gamma-variant transfer function. We find consistent predictions across the cortex, which are best at low frequency (0.009–0.08 Hz). Furthermore, we show that the relationship between modality connectivity strengths varies by region. Our approach links cell-type-specific optical measurements of activity to the most widely used method for assessing human brain function.

全面了解大脑功能需要具有互补优势的多种成像方式。我们提出了一种同时进行宽场光学和功能磁共振成像的方法。通过合并这些方式，我们可以同时获得大脑活动的全脑血氧水平依赖性 (BOLD) 和全皮层钙敏感荧光测量。在转基因鼠模型中，我们使用优化伽马变体传递函数的模型显示钙可以预测 BOLD 信号。我们发现整个皮层的预测一致，在低频（0.009-0.08 Hz）时效果最好。此外，我们表明模态连接强度之间的关系因地区而异。