Abstract

The objective of the present work was to develop biodegradable polymeric films (starch-PBAT) as support for the immobilization of lipases using sodium montmorillonite (MMT) as a reinforcing agent (2% w/w) and itaconic acid (IA − 0.5–1.5% w/w) as a compatibilizing agent. The films were produced through a two steps blow-extrusion. The addition of MMT increased the tensile strength and Tg of the films, while the presence of IA made the films more flexible, reducing their Tg. Lipases from Burkholderia cepacia LTEB11 were immobilized in the films by the adsorption method. The ester yield (% of ethyl oleate synthesis) has shown best results (96%, 6 h) for immobilized enzyme in the MMT film and six cycles of reuse were carried out until a reduction of 50% in the catalytic activity of the enzyme.

摘要

本工作的目的是开发可生物降解的聚合物薄膜（淀粉-PBAT）作为使用蒙脱石钠（MMT）作为增强剂（2% w/w）和衣康酸（IA - 0.5-1.5）固定脂肪酶的支持% w/w ) 作为增容剂。薄膜通过两步吹塑挤出生产。MMT的加入增加了薄膜的拉伸强度和Tg，而IA的存在使薄膜更柔韧，降低了它们的Tg。洋葱伯克霍尔德菌的脂肪酶LTEB11 通过吸附法固定在薄膜中。对于 MMT 膜中的固定化酶，酯产量（油酸乙酯合成的百分比）显示出最佳结果（96%，6 小时），并且进行了六次重复使用，直到酶的催化活性降低 50%。