当前位置: X-MOL 学术Electrophoresis › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Determination of complex type free, non‐conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency
Electrophoresis ( IF 3.0 ) Pub Date : 2020-10-31 , DOI: 10.1002/elps.202000254
Bence Mátyás 1, 2 , Júlia Singer 3 , Máté Szarka 4, 5, 6 , Daniel A Lowy 1, 2, 7 , Boglárka Döncző 5 , Péter Makleit 8 , Virgilio E Failoc-Rojas 9 , Andrés Ramirez 10 , Pedro Martínez 10 , Zsolt Sándor 2, 8 , Ida Kincses 8 , András Guttman 4, 11
Affiliation  

Although knowledge on glycan biosynthesis and processing is continuously maturing, there are still a limited number of studies that examine biological functions of N‐glycan structures in plants, which remain virtually unknown. Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8‐aminopyrene‐1,3,6‐trisulfonic acid (APTS)‐labeled complex type free oligosaccharides is reported. While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS‐labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE‐LIF). Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org).

中文翻译:

确定番茄木质部汁液中无复合类型的非共轭寡糖葡萄糖单位值,用于早期检测营养缺乏

尽管关于聚糖生物合成和加工的知识不断成熟,但仍然有数量有限的研究来检查N 的生物功能-植物中的聚糖结构,这实际上仍然是未知的。在这里,报告了作物营养(氮)缺乏症状与 8-氨基芘-1,3,6-三磺酸(APTS)标记的复合型游离寡糖变化之间的统计相关性。虽然缺乏症状是通过多光谱图像和凯氏定氮消化来预测的,但 APTS 标记的复合型游离寡糖是通过其在番茄木质部汁液中的葡萄糖单位 (GU) 值来识别的,使用毛细管电泳和激光诱导荧光检测 (CE-LIF)。鉴于从植物中获得的结构数量有限,在文献中存档,未来打算创建一个开放获取的数据库,其中包含有希望的指标,即可能导致植物中营养缺乏引起压力的聚糖结构(http :
更新日期:2020-10-31
down
wechat
bug