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Role of RpoS in stress resistance, biofilm formation and quorum sensing of Shewanella baltica
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-11-20 , DOI: 10.1111/lam.13424
C. Zhang 1 , C. Wang 1 , A.‐N. Jatt 2 , H. Liu 1 , Y. Liu 1
Affiliation  

Shewanella baltica is one of the most important bacterial species contributing to spoilage of seafood. Principally, RpoS has been recognized as the central regulator of stress resistance in many bacterial species. However, little is known about the role of RpoS in S. baltica. In this study, an rpoS mutant of S. baltica was constructed and analyzed for its functions. The results showed that the survival rate of rpoS mutant decreased when treated with heat, ethanol and H2 O2, while increased the resistance to NaCl. Moreover, RpoS promoted the biofilm formation of S. baltica at 30 ℃, while declined at 4 ℃. Interestingly, the rpoS deficient mutant showed increased swimming motility. Furthermore, the results revealed that the production of quorum-sensing (QS) signals such as cyclo-(L-Pro-L-Leu) and cyclo-(L-Pro-L-Phe) reduced in rpoS mutant. Mainly, rpoS positively regulated QS response regulators, as the expression of all luxR genes in rpoS mutant significantly decreased relative to wild type. This study reveals that RpoS is a major regulator involved in stress responses, biofilm formation and quorum sensing system in S. baltica. The present work provides significant information for the control of microbiological spoilage of seafood.

中文翻译:

RpoS 在 Shewanella baltica 的抗逆性、生物膜形成和群体感应中的作用

Shewanella baltica 是导致海鲜腐败的最重要的细菌种类之一。原则上,RpoS 已被公认为许多细菌物种抗逆性的中心调节剂。然而,关于 RpoS 在 S. baltica 中的作用知之甚少。在这项研究中,构建了 S. baltica 的 rpoS 突变体并分析了其功能。结果表明,rpoS突变体在加热、乙醇和H2O2处理下存活率降低,而对NaCl的抗性增加。此外,RpoS在30 ℃促进S. baltica生物膜形成,而在4 ℃下降。有趣的是,rpoS 缺陷突变体表现出增加的游泳运动。此外,结果表明,在 rpoS 突变体中,诸如环-(L-Pro-L-Leu) 和环-(L-Pro-L-Phe) 等群体感应 (QS) 信号的产生减少。主要是,rpoS 正向调节 QS 反应调节因子,因为 rpoS 突变体中所有 luxR 基因的表达相对于野生型显着降低。该研究表明,RpoS 是波罗的海海藻应激反应、生物膜形成和群体感应系统的主要调节因子。目前的工作为控制海鲜的微生物腐败提供了重要信息。
更新日期:2020-11-20
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