Rapid identification of the invasive Small hive beetle (Aethina tumida) using LAMP,Pest Management Science

当前位置： X-MOL 学术 › Pest Manag. Sci. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Rapid identification of the invasive Small hive beetle (Aethina tumida) using LAMP
Pest Management Science ( IF 3.8 ) Pub Date : 2020-11-02 , DOI: 10.1002/ps.6168
Sally Ponting ₁ , Victoria Tomkies ₁ , Kirsty Stainton _{1,

2}

Affiliation

Small hive beetle (SHB), Aethina tumida is an invasive pest of managed honey bees and has invaded Europe from Africa. The main risk point identified for incursions of this pest into the UK is imports of package bees and queens. Surveillance of this pest, carried out by the National Bee Unit, involves monitoring imports of live bees and routine screening of sentinel apiaries around the UK through collection of hive debris for visual inspection of pests. Currently, no molecular methods are used for screening hive debris for this pest in the UK. A new invasion of this pest would likely occur at low levels and might present with partial specimens broken up in the hive or eggs from the beetle, difficult to identify by visual inspection. Therefore, we sought to develop a fast and sensitive method for detecting SHB in hive debris by developing a loop‐mediated isothermal amplification (LAMP) assay.

中文翻译：

使用LAMP快速鉴定侵入性小蜂虫（Aethina tumida）

小蜂房甲虫（Athina ethida）是管理蜜蜂的一种入侵性害虫，已从非洲入侵欧洲。确定将这种有害生物入侵到英国的主要风险点是蜜蜂和皇后package的进口。由国家蜜蜂部门对这种害虫进行监视，包括监测活蜂的进口，并通过收集蜂巢碎片对害虫进行目视检查，对英国各地的定点蜂进行常规筛查。目前，在英国，没有分子方法用于筛查这种有害生物的蜂巢碎片。这种有害生物的新入侵很可能发生在低水平，并且可能会在蜂巢中分解出部分标本或从甲虫中分裂出卵，很难通过肉眼检查来识别。因此，

更新日期：2020-11-02

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11