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Wavefront-sensorless adaptive optics with a laser-free spinning disk confocal microscope
Journal of Microscopy ( IF 1.5 ) Pub Date : 2020-10-30 , DOI: 10.1111/jmi.12976
Syed Asad Hussain 1 , Toshiki Kubo 2 , Nicholas Hall 3 , Dalia Gala 4 , Karen Hampson 1 , Richard Parton 4 , Mick A Phillips 3 , Matthew Wincott 1 , Katsumasa Fujita 2 , Ilan Davis 4 , Ian Dobbie 3 , Martin J Booth 1
Affiliation  

Adaptive optics is being applied widely to a range of microscopies in order to improve imaging quality in the presence of specimen-induced aberrations. We present here the first implementation of wavefront-sensorless adaptive optics for a laser-free, aperture correlation, spinning disk microscope. This widefield method provides confocal-like optical sectioning through use of a patterned disk in the illumination and detection paths. Like other high-resolution microscopes, its operation is compromised by aberrations due to refractive index mismatch and variations within the specimen. Correction of such aberrations shows improved signal level, contrast and resolution.

中文翻译:

无激光旋转圆盘共聚焦显微镜的无波前传感器自适应光学系统

自适应光学正被广泛应用于一系列显微镜,以在存在标本引起的像差的情况下提高成像质量。我们在这里展示了第一个用于无激光、孔径相关、旋转圆盘显微镜的无波前传感器自适应光学系统的实现。这种宽视场方法通过在照明和检测路径中使用图案化圆盘提供类似共焦的光学切片。与其他高分辨率显微镜一样,由于折射率不匹配和标本内的变化,它的操作会受到像差的影响。此类像差的校正显示出改进的信号电平、对比度和分辨率。
更新日期:2020-10-30
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