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Effects of Greek juniper (Juniperus excelsa) extract on immune responses and disease resistance against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss)
Journal of Fish Diseases ( IF 2.2 ) Pub Date : 2020-10-31 , DOI: 10.1111/jfd.13293
Soner Bilen 1 , Selcuk Ispir 1 , Osman Nezih Kenanoglu 1 , Yiğit Taştan 1 , Kerim Güney 2 , Ertugrul Terzi 1
Affiliation  

This study investigated the effects of Greek juniper extract on immune responses of rainbow trout. In this experiment, 4 doses [0 (Control), 1 (J1), 4 (J4) and 8 (J8) mg/kg] of the extract were administered orally using an oral gavage twice a day for 14 days. Immune responses were measured on 7th and 14th days. On 14th day, Yersinia ruckeri was injected intraperitoneally to all fish of all groups. On 14th day, ORP in fish of J1 group increased significantly. Lysozyme activity (LA) was increased in J8 group on 7th day (p < .05). On 14th day, a significant decrease was determined in J1 and J4 treatments in LA. Myeloperoxidase activity was significantly decreased in all groups irrespective of sampling times (p < .05). Interleukin (IL)‐1β was significantly elevated in fish of J8 group on 7th day. IL‐8 increased in fish of J8 and J4 groups on 7th day of the study. IL‐12 gene expression was significantly up‐regulated in J8 fish group on 7th day, and in J4 fish group on 14th day. Survival rate was higher in J8 treatment compared to the control and other treatments (p < .05). The results suggest that Juniperus excelsa provides protection against Y. ruckeri in rainbow trout.

中文翻译:

希腊杜松提取物(Juniperus excelsa)提取物对虹鳟(Oncorhynchus mykiss)的免疫反应和抗立克次体的疾病抗性的影响

这项研究调查了希腊杜松提取物对虹鳟鱼免疫反应的影响。在该实验中,每天两次通过口服管饲法口服给药4剂量[0(对照),1(J1),4(J4)和8(J8)mg / kg)提取物,持续14天。在第7天和第14天测量免疫应答。在第14天,腹腔注射耶尔森氏菌至所有组的所有鱼。第14天,J1组鱼的ORP显着增加。J8组的溶菌酶活性(LA)在第7天升高(p  <.05)。在第14天,在洛杉矶的J1和J4治疗中确定了显着下降。所有组的髓过氧化物酶活性均显着降低,而与采样时间无关(p <.05)。第7天,J8组鱼的白介素(IL)-1β显着升高。在研究的第7天,J8和J4组的鱼中IL-8升高。J8鱼组第7天和J4鱼组第14天IL-12基因表达显着上调。与对照组和其他治疗相比,J8治疗的存活率更高(p  <.05)。结果表明,刺柏棕竹提供保护以防止Y.耶尔森虹鳟鱼。
更新日期:2020-10-31
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