Regulation of circadian behavioural output via clock‐responsive miR‐276b,Insect Molecular Biology

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Regulation of circadian behavioural output via clock‐responsive miR‐276b
Insect Molecular Biology ( IF 2.3 ) Pub Date : 2020-11-01 , DOI: 10.1111/imb.12679
R Zhang ₁ , J Du ₁ , X Zhao ₁ , L Wei ₂ , Z Zhao ₁

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Growing evidence indicates that microRNAs play numerous important roles. However, the roles of some microRNAs involved in regulation of circadian rhythm and sleep are still not well understood. In this study, we show that the miR‐276b is essential for maintaining both sleep and circadian rhythm by targeting tim, npfr1 and DopR1 genes, with miR‐276b deleted mutant flies sleeping more, and vice versa in miR‐276b overexpressing flies. Through analysing its promoter, we found that mir‐276b is responsive to CLOCK and regulates circadian rhythm through the negative feedback loop of the CLK/CYC‐TIM/PER. Furthermore, miR‐276b is broadly expressed in the clock neurons and the central complexes such as the mushroom body and the fan‐shape body of Drosophila brain, in which up‐regulation of miR‐276b in tim, npfr1 and DopR1 expressing tissues significantly causes sleep decreases. This study clarifies that the mir‐276b is very important for participating in regulation of circadian rhythm and sleep.

中文翻译：

通过时钟响应 miR-276b 调节昼夜节律行为输出

越来越多的证据表明 microRNA 发挥着许多重要作用。然而，一些参与调节昼夜节律和睡眠的 microRNA 的作用仍不清楚。在这项研究中，我们证明了的miR-276B是通过靶向维持睡眠和昼夜节律必不可少恬，npfr1和DopR1基因，用的miR-276B的缺失突变体果蝇睡眠时间，反之亦然的miR-276B过度表达的苍蝇。通过分析其启动子，我们发现mir-276b响应 CLOCK，并通过 CLK/CYC-TIM/PER 的负反馈回路调节昼夜节律。此外，miR-276b在时钟神经元和中央络合物被广泛表达，如蘑菇体和的扇形体果蝇脑，其中的上调的miR-276B中添，npfr1和DopR1表达组织显著使睡觉降低。这项研究阐明了mir-276b对于参与调节昼夜节律和睡眠非常重要。

更新日期：2021-01-06

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