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TaPUB15, a U‐Box E3 ubiquitin ligase gene from wheat, enhances salt tolerance in rice
Food and Energy Security ( IF 4.0 ) Pub Date : 2020-10-31 , DOI: 10.1002/fes3.250 Qiaoru Li 1 , Bo Li 1 , Jingyi Wang 1 , Xiaoping Chang 1 , Xinguo Mao 1 , Ruilian Jing 1
Food and Energy Security ( IF 4.0 ) Pub Date : 2020-10-31 , DOI: 10.1002/fes3.250 Qiaoru Li 1 , Bo Li 1 , Jingyi Wang 1 , Xiaoping Chang 1 , Xinguo Mao 1 , Ruilian Jing 1
Affiliation
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Plant U‐box (PUB) E3 ubiquitin ligases play essential roles in response to environmental stresses and hormone signaling, but little is known about them in wheat (Triticum aestivum L.). We characterized a U‐box E3 ubiquitin ligase gene, TaPUB15, which is conserved in wheat cultivars. TaPUB15 was expressed in multiple tissues, and the expression was much higher in roots than in other tissues. Transcription of TaPUB15 was induced by salt, abscisic acid, 4°C, and polyethylene glycol (PEG). Overexpression of TaPUB15‐D led to improve salt tolerance in transgenic rice, along with more roots in transgenic rice. Ion flux assays showed that transgenic rice plants had improved salt tolerance and maintained low Na+/K+ ratios under salinity stress. Gene expression assays indicated that several salt stress‐related genes were significantly upregulated in transgenic rice relative to the wild type. These results from transgenic rice were also verified in transgenic Arabidopsis. Our data showed that TaPUB15 plays crucial roles in enhancing salt tolerance in crop plants and served a valuable gene resource for promoting the breeding of salt‐tolerant wheat in the future.
中文翻译：
TaPUB15是小麦的U-Box E3泛素连接酶基因，可增强水稻的耐盐性
植物U-box（PUB）E3泛素连接酶在响应环境压力和激素信号方面起着至关重要的作用，但对于小麦（小麦）却知之甚少。我们表征了一个U-box E3泛素连接酶基因TaPUB15，该基因在小麦品种中是保守的。TaPUB15在多种组织中表达，并且在根中的表达远高于其他组织。TaPUB15的转录是由盐，脱落酸，4°C和聚乙二醇（PEG）诱导的。TaPUB15-D的过度表达提高了转基因水稻的耐盐性，并增加了转基因水稻的根系。离子通量分析表明转基因水稻植物具有更高的耐盐性并保持低钠盐胁迫下的+ / K +比率。基因表达分析表明，与野生型相比，转基因水稻中几个盐胁迫相关基因显着上调。转基因水稻的这些结果在转基因拟南芥中也得到证实。我们的数据表明，TaPUB15在增强农作物的耐盐性中起着关键作用，并为将来促进耐盐小麦的育种提供了宝贵的基因资源。
更新日期：2020-10-31
中文翻译：
TaPUB15是小麦的U-Box E3泛素连接酶基因，可增强水稻的耐盐性
植物U-box（PUB）E3泛素连接酶在响应环境压力和激素信号方面起着至关重要的作用，但对于小麦（小麦）却知之甚少。我们表征了一个U-box E3泛素连接酶基因TaPUB15，该基因在小麦品种中是保守的。TaPUB15在多种组织中表达，并且在根中的表达远高于其他组织。TaPUB15的转录是由盐，脱落酸，4°C和聚乙二醇（PEG）诱导的。TaPUB15-D的过度表达提高了转基因水稻的耐盐性，并增加了转基因水稻的根系。离子通量分析表明转基因水稻植物具有更高的耐盐性并保持低钠盐胁迫下的+ / K +比率。基因表达分析表明，与野生型相比，转基因水稻中几个盐胁迫相关基因显着上调。转基因水稻的这些结果在转基因拟南芥中也得到证实。我们的数据表明，TaPUB15在增强农作物的耐盐性中起着关键作用，并为将来促进耐盐小麦的育种提供了宝贵的基因资源。
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