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Optimization, kinetic, and scaling-up of solvent-free lipase-catalyzed synthesis of ethylene glycol oleate emollient ester
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-11-02 , DOI: 10.1002/bab.2067
Eloise de Sousa Cordeiro 1 , Rosana Oliveira Henriques 1 , Eduardo Monteiro Deucher 1 , Débora de Oliveira 1 , Lindomar Alberto Lerin 2 , Agenor Furigo 1
Affiliation  

The use of enzymatic catalysts is an alternative to chemical catalysts as they can help to obtain products with less environmental impact, considered sustainable within the concept of green chemistry. The optimization, kinetic, lipase reuse, and scale-up of enzymatic production of ethylene glycol oleate in the batch mode were carried out using the NS 88011 lipase in a solvent-free system. For the optimization step, a 23 Central Composite Design was used and the optimized condition for the ethylene glycol oleate production, with conversions above 99%, was at 70 °C, 600 rpm, substrates molar ratio of 1:2, 1 wt% of NS 88011 in 32 H of reaction. Kinetic tests were also carried out with different amounts of enzyme, and it showed that by decreasing the amount of the enzyme, the conversion also decreases. The lipase reuse showed good conversions until the second cycle of use, after which it had a progressive reduction reaching 83% in the fourth cycle of use. The scale-up (ninefold increase) showed promising results, with conversion above 99%, achieving conversions similar to small-scale reactions. Therefore, this work proposed an environmentally safe route to produce an emollient ester using a low-cost biocatalyst in a solvent-free system.

中文翻译:

无溶剂脂肪酶催化合成乙二醇油酸酯润肤酯的优化、动力学和放大

酶催化剂的使用是化学催化剂的替代品,因为它们可以帮助获得对环境影响较小的产品,在绿色化学的概念中被认为是可持续的。使用 NS 88011 脂肪酶在无溶剂系统中进行了优化、动力学、脂肪酶再利用和批量模式下乙二醇油酸酯酶促生产的放大。对于优化步骤,a 2 3采用中心复合设计,在 70 °C、600 rpm、底物摩尔比为 1:2、1 wt% 的 NS 88011 反应时间为 32 H 时,生产乙二醇油酸酯的优化条件为 99% 以上,转化率高于 99% . 还对不同量的酶进行了动力学测试,结果表明,通过减少酶的量,转化率也会降低。脂肪酶再利用在第二个使用周期之前显示出良好的转化率,之后在第四个使用周期中逐渐减少达到 83%。放大(增加九倍)显示出可喜的结果,转化率高于 99%,实现了类似于小规模反应的转化率。因此,这项工作提出了一种环境安全的路线,在无溶剂系统中使用低成本的生物催化剂生产润肤酯。
更新日期:2020-11-02
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