ABSTRACT The effect of solid-state fermentation (SSF) of avocado seed with A. niger GH1 on the release of bioactive compounds with antioxidant capacity was studied. The growing conditions of the fungus were optimized. Subsequently, The effect of sterile water and pontecorvo culture medium was evaluated in the SSF. The optimal conditions for SSF were particle size of 2.5 mm, 60 % of humidity, and 120 h. In the assessments of antioxidant capacity by the ABTS and DPPH methods, the increase with respect to the initial values was 33 and 77 % with water and 28 and 42 % with culture medium, respectively. The analysis HPLC showed degradation of catechin and epicatechin polymers, particularly procyanidin B2 dimers resulting in the accumulation of monomers. The ability of the fungus to degrade compounds present in the avocado seed was decisive to enhance the antioxidant capacity. This degradation was related to increased cellulase and xylanase activity.

中文翻译：

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固态发酵——辅助从哈斯鳄梨种子中提取生物活性化合物

摘要研究了鳄梨种子与 A. niger GH1 的固态发酵 (SSF) 对释放具有抗氧化能力的生物活性化合物的影响。优化了真菌的生长条件。随后，在 SSF 中评估了无菌水和 pontecorvo 培养基的效果。SSF 的最佳条件是粒径为 2.5 毫米、湿度为 60% 和 120 小时。在通过 ABTS 和 DPPH 方法评估抗氧化能力时，与初始值相比，水增加了 33% 和 77%，培养基增加了 28% 和 42%。分析 HPLC 显示儿茶素和表儿茶素聚合物降解，尤其是原花青素 B2 二聚体，导致单体积累。真菌降解鳄梨种子中化合物的能力对于增强抗氧化能力具有决定性意义。这种降解与纤维素酶和木聚糖酶活性增加有关。