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The European badger, Meles meles, as a new host for Trichinella britovi in Romania
Veterinary Parasitology ( IF 2.6 ) Pub Date : 2020-11-02 , DOI: 10.1016/j.vetpar.2020.109301
Zsolt Boros 1 , Angela Monica Ionică 2 , Georgiana Deak 1 , Andrei Daniel Mihalca 1 , Gabriel Bogdan Chisamera 3 , Adriana Györke 1 , Călin Mircea Gherman 1 , Vasile Cozma 4
Affiliation  

The European badger, Meles meles (Carnivora, Mustelidae) is a widespread opportunistic omnivorous mammal. Its food spectrum comprises a wide variety of plants and small animals, occasionally including rodents, such as mice or rats. Considering that rodents are known to play a key role in the life cycle of Trichinella spp., the aims of this study were to investigate the occurrence of these parasites in badgers from Romania and to identify the species. Overall, 61 badgers originating from 14 counties were examined by trichinoscopy and artificial digestion. For species determination, the positive muscle samples, and the larvae recovered from the artificial digestion were used for DNA isolation, and further processed by multiplex PCR. A single badger, originating from Sibiu County, Central Romania, was found positive for Trichinella spp. Five cysts were identified using trichinoscopy: four in the diaphragm and one in the foreleg muscles. Artificial digestion revealed an infection rate of 70 larvae/100 g of muscle. The PCR indicated the occurrence of T. britovi, which is the most commonly detected species in wild carnivores in temperate areas. Although T. britovi has previously been reported in Romania, this represents the first report of its occurrence in the European badger in the country. However, the low prevalence indicates a minor reservoir role of this species.



中文翻译:

欧洲badge Meles meles作为罗马尼亚Trichinella britovi的新主人

欧洲badge,Meles meles(食肉动物,鼬科)是一种广泛的机会性杂食性哺乳动物。它的食物范围包括各种各样的植物和小型动物,偶而包括啮齿类动物,例如小鼠或大鼠。考虑到啮齿动物在旋毛虫的生命周期中起着关键作用spp。,这项研究的目的是调查罗马尼亚badge中这些寄生虫的发生并鉴定其种类。总体而言,通过旋毛虫镜和人工消化检查了来自14个县的61个rs。为了进行物种确定,将阳性肌肉样品和从人工消化中回收的幼虫用于DNA分离,并通过多重PCR进行进一步处理。发现一种源自罗马尼亚中部锡比乌县的badge,对旋毛虫属呈阳性。使用三角镜检查法鉴定出五个囊肿:在diaphragm肌中四个,在前腿肌肉中一个。人工消化显示,幼虫/ 100 g肌肉的感染率。PCR表明发生了布里托氏杆菌是温带地区野生食肉动物中最常见的物种。尽管以前曾在罗马尼亚报道过布氏烟草(T. britovi),但这是该病毒在该国欧洲badge中的首次报道。但是,低流行率表明该物种的储层作用较小。

更新日期:2020-11-06
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