Tracking the genetic diversity and spread of swine influenza viruses (SIVs) in commercial swine farms is central for control and to reduce the potential emergence of SIV reassortants. We analyzed the diversity of SIVs in nasal washes or oral fluids from commercial swine farms in North Carolina using influenza M qRT-PCR and hemagglutinin (HA) and neuraminidase (NA) subtyping. We found a predominance of H1 HAs and N2 NAs in the samples examined. The majority of the H1 HAs could be further classified into gamma and delta subclusters. We also identified HAs of the H1 alpha cluster, and those of human novel pandemic origin. Glycan binding profiles from a representative subset of these viruses revealed broad α2,6 sialylated glycan recognition, though some strains exhibited the ability to bind to α2,3 sialic acid. These data show that SIV surveillance can aid our understanding of viral transmission dynamics and help uncover the diversity at the human-swine interface.

跟踪商业猪场中猪流感病毒（SIV）的遗传多样性和传播对于控制和减少SIV重排子的潜在出现至关重要。我们使用流感M qRT-PCR以及血凝素（HA）和神经氨酸酶（NA）亚型分析了北卡罗来纳州商业猪场洗鼻液或口服液中SIV的多样性。我们在所检查的样品中发现了H1 HA和N2 NA的优势。大多数H1 HA可以进一步分类为γ和δ子类。我们还确定了H1 alpha簇的HA，以及人类新型大流行病的HA。来自这些病毒的代表性子集的聚糖结合谱显示出广泛的α2，6唾液酸化聚糖识别，尽管一些菌株表现出结合α2，3唾液酸的能力。