There has been rapid growth in the use of larval zebrafish as a complementary vertebrate model for drug discovery, abuse liability and pharmacological toxicology, resulting in a huge increase in zebrafish facilities worldwide. However, many research groups working with zebrafish do not typically report the pH of husbandry conditions in methodologies, nor are the pH of drug treatments reported in many research articles. This unknown factor can be a major contributor in the differential effects of drug treatments. Therefore, as a case study, we tested the impact of altering pH of several drugs of abuse and assessed locomotor changes associated with a single drug concentration delivered at different pHs. We found that a change of a single pH unit, within the pH ranges commonly used in zebrafish husbandry, was enough to alter locomotor activity at a fixed drug concentration. Many pharmacological agents are dependent on environmental factors, such as pH, to determine bioavailability. Efficaciousness for many classes of drug is dependent on their ionization state in which shifts towards uncharged species can influence the easy of a drug crossing biological membranes. Thus, we urge users to report pH in husbandry methods and drug treatments to improve replicability and inter-study comparisons.

幼虫斑马鱼作为药物发现，滥用责任和药理毒理学的补充脊椎动物模型的使用迅速增长，导致全世界斑马鱼设施的大量增加。但是，许多使用斑马鱼的研究小组通常没有在方法学中报告饲养条件的pH值，许多研究文章中也没有报道药物治疗的pH值。这个未知因素可能是药物治疗差异作用的主要因素。因此，作为案例研究，我们测试了几种滥用药物改变pH值的影响，并评估了与在不同pH值下递送的单一药物浓度相关的运动变化。我们发现，在斑马鱼饲养中常用的pH范围内，单个pH单位发生了变化，在固定的药物浓度下足以改变运动活性。许多药物依赖于环境因素（例如pH）来确定生物利用度。许多类药物的有效性取决于其电离状态，在这种状态下，向不带电物质的迁移会影响药物穿过生物膜的难易程度。因此，我们敦促用户在饲养方法和药物治疗中报告pH值，以提高可复制性和研究间的比较。