The heat shock protein family 70 (Hsp70) comprises chaperone proteins that play major multiple roles in Plasmodium asexual and sexual development. In this study, we analyzed the expression of Hsp70-1 in gametocytes, gametes, zygotes, and its participation in ookinete formation and their transition into oocysts. A monoclonal antibody against recombinant Hsp70-1 revealed its presence in zygotes and micronemes of ookinetes. Compared to wild type parasites, Hsp70-1 knockout ookinetes produced fewer oocysts in Plasmodium-susceptible Anopheles albimanus mosquitoes. This may indicate a defective transformation of ookinetes into oocysts in the absence of Hsp70-1. The presence of this protein in micronemes suggests its participation in mosquito infection, probably aiding to the adequate structural conformation of proteins in charge of motility, recognition and invasion of the insect midgut epithelium.

热休克蛋白家族70（Hsp70）包含伴侣蛋白，这些蛋白在疟原虫无性和性发育中起主要多重作用。在这项研究中，我们分析了Hsp70-1在配子细胞，配子，受精卵中的表达，以及它参与卵子形成和向卵囊过渡的过程。抗重组Hsp70-1的单克隆抗体显示其存在于合子的合子和微nemene中。与野生型寄生虫相比，Hsp70-1基因敲除的钩虫在疟原虫易感按蚊中产生的卵囊更少蚊子。这可能表明在不存在Hsp70-1的情况下，融合蛋白转化为卵囊的缺陷。这种蛋白质在微nemene中的存在表明它参与了蚊子的感染，可能有助于负责蠕动，识别和侵袭昆虫中肠上皮的蛋白质的适当结构构象。