Soybean (Glycine max L.) is among the most economically important legumes that provide more than 1/4 of food (for man) and animal feed. However, its yield is comparatively low, most especially under drought stress. The aim of this study therefore was to assess the ability of Rhizobium spp. and mycorrhizal fungi to enhance the yield, seed size and fatty acid content of soybean grown under semi-arid environment. Rhizobium sp. strain R1 was found to possess nitrogen-fixing gene coniferyl aldehyde dehydrogenase function while Rhizobium cellulosilyticum strain R3 was found to have nitrogen-fixing genes cysteine desulfurase SufS and cysteine desulfurase IscS activity. Soybean (Glycine max L) seeds inoculated with Rhizobium spp. and mycorrhizal fungi were cultivated in soil exposed to drought stress. Rhizobium spp. inoculation and mycorrhization alleviate drought stress and increase yield, size and fat content of soybean seeds.. This increase in the aboveground parameters was accompanied with an increase in belowground mycorrhizal spore number, percentage root mycorrhization and aboveground shoot relative water content (RWC) in the dually inoculated (R1 + R3MY) soybean plants. In particular, the dually inoculated (R1 + R3MY) soybean plants revealed 34.3 g fresh weight, 15.1 g dry weight and soybean plants singly inoculated with Rhizobium sp. strain R1 (R1) produced more large seeds with 12.03 g dry weight. The non-inoculated (control) seeds contained a higher percentage of moisture content compared to the microbially amended seeds while seeds co-inoculated with Rhizobium cellulosilyticum strain R3 and mycorrhizal consortium revealed the highest percent (8.4%) of fat. Several fatty acids that are of significant health benefits to humans were observed in the soybean seeds. In order to gain insights into the bacterial communities of rhizospheric soil collected at different stages of soybean growth, class-based Heat-map analysis was performed on the Miseq sequenced data. The core bacteria that were found in the rhizospheric soil were Verrumicrobia, Proteobacteria, Gemmatimonadetes, Firmicutes, Cyanobacteria, Chloroflexi, Bacteroidetes, Actinobacteria, Acidobacteria, Planctomycetes, Deinococcus_thermus and Nitrospira suggesting that the rhizobia and fungi used in this study can also improve soil microbial diversity.

大豆 ( Glycine max L.) 是经济上最重要的豆类之一，提供超过 1/4 的食物（人类）和动物饲料。然而，其产量相对较低，尤其是在干旱胁迫下。因此，本研究的目的是评估根瘤菌的能力。和菌根真菌，以提高半干旱环境下种植的大豆的产量、种子大小和脂肪酸含量。根瘤菌属。发现菌株R1具有固氮基因松柏醛脱氢酶功能，而发现纤维素根瘤菌菌株R3具有固氮基因半胱氨酸脱硫酶SufS和半胱氨酸脱硫酶IscS活性。接种根瘤菌的大豆 ( Glycine max L) 种子。和菌根真菌在暴露于干旱胁迫的土壤中培养。根瘤菌属接种和菌根化缓解干旱胁迫，提高大豆种子的产量、大小和脂肪含量。地上部参数的增加伴随着地下菌根孢子数、根菌根百分比和地上部相对含水量（RWC）的增加。双重接种（R1 + R3MY）大豆植物。特别是，双重接种（R1 + R3MY）的大豆植物显示出34.3克鲜重，15.1克干重，而单次接种根瘤菌的大豆植物则显示出34.3克鲜重，15.1克干重。菌株 R1 (R1) 产生更大的种子，干重为 12.03 克。 与微生物改良的种子相比，未接种（对照）的种子含有更高百分比的水分含量，而与解纤根瘤菌菌株 R3 和菌根聚生体共同接种的种子显示出最高的脂肪百分比（8.4%）。在大豆种子中发现了几种对人类健康有显着益处的脂肪酸。为了深入了解大豆生长不同阶段收集的根际土壤的细菌群落，对 Miseq 测序数据进行了基于类别的热图分析。根际土壤中发现的核心细菌为疣微菌门、变形菌门、芽孢杆菌门、厚壁菌门、蓝细菌门、绿柔菌门、拟杆菌门、放线菌门、酸杆菌门、浮霉菌门、嗜热奇球菌门和硝化螺菌门，表明本研究中使用的根瘤菌和真菌也可以改善土壤微生物多样性。 。