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Genetic diversity of Salvia species from Turkey assessed by microsatellite markers
Journal of Applied Research on Medicinal and Aromatic Plants ( IF 3.8 ) Pub Date : 2020-11-02 , DOI: 10.1016/j.jarmap.2020.100281
Nadire P. Bahadirli , Filiz Ayanoglu

Salvia is one of the most important medicinal plant genus in the Lamiaceae family. The genetic diversity of 85 genotypes belonging to 20 species from the native flora of the Hatay, province of south-central part of Turkey, was analyzed using six microsatellite markers. These markers (SoUZ022, SoUZ024, SoUZ025, SoUZ026, SoUZ027, and SoUZ028) were derived from S. officinalis and were tested in this study to see their cross-species amplification in different Salvia species. Primer screenings results were found as follows; the total number of alleles (Na) was 160, the observed heterozygosity (HO) values were ranged from 0.871 to 0.976, the expected heterozygosity (HE) values ranged from 0.871 to 0.976, the polymorphism information content (PIC) ranged from 0.764 to 0.947, and the number of alleles ranged from 19 to 34. The genetic diversity of the flora presented in a dendrogram. In the dendrogram, all the genotypes collected from 85 locations are clustered heterogeneously into four main groups. S. fruticosa was found to be more distant species than the other species in the study. S. aramiensis and S. aucheri were found to be the closest species.



中文翻译:

通过微卫星标记评估来自土耳其的丹参物种的遗传多样性

丹参是唇形科中最重要的药用植物之一。使用六个微卫星标记分析了来自土耳其南部中部省Hatay本地植物区系的20个物种的85个基因型的遗传多样性。这些标记(SoUZ022,SoUZ024,SoUZ025,SoUZ026,SoUZ027和SoUZ028)从衍生鼠尾草和在本研究中进行测试以看它们的交叉物种在不同的放大丹参物种。引物筛选结果如下:等位基因总数(Na)为160,观察到的杂合度(H O)值范围为0.871至0.976,预期杂合度(H E)值的范围为0.871至0.976,多态性信息含量(PIC)的范围为0.764至0.947,等位基因的数量范围为19至34。树状图中显示的菌群遗传多样性。在树状图中,从85个位置收集的所有基因型均异质地分为四个主要组。在研究中发现金黄色葡萄球菌比其他物种更远。S. aramiensisS. aucheri被认为是最接近的物种。

更新日期:2020-11-02
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