Since European maximum guidance values of mycotoxins are only available for feed, mycotoxin exposure in animals is mainly monitored by feed analysis. However, proper sample collection is needed to ensure reliable results, because of uneven distributions and disproportional spread of mycotoxins in feed which can hamper the evaluation of mycotoxin exposure in animals. A cross-sectional study was performed on 40 randomly selected broiler farms in Belgium. During a farm visit at the animal’s age of 28 days, a pooled feed sample at the beginning and the end of the feed line was collected. Feed samples were analyzed by a validated multi-mycotoxin LC-MS/MS method. Moreover, serum samples were collected from 10 randomly selected chickens per farm. Serum concentrations of mycotoxins and major in vivo phase I metabolites were analyzed quantitatively, while the presence of phase II metabolites was determined in a qualitative approach by an UPLC-HRMS method. Deoxynivalenol (DON) was the most frequently occurring mycotoxin, being present in 74% of the feed samples, with an average concentration of 270 ± 171 μg/kg and a maximum concentration of 751 μg/kg in positive samples. Also the acetylated forms 3- and 15-acetyldeoxynivalenol (3 and 15ADON) were present in half of the samples, however, at lower concentrations (8 ± 3 μg 3ADON and 10 ± 7 μg 15ADON/kg). Only in 17.5 % of the farms DON was detected in serum samples at a mean serum concentration and standard deviation (SD) of 11 ± 19 ng/mL. The maximum serum concentration of 49 ng DON/mL was detected in broilers which were fed a diet that was contaminated with 191 μg DON/kg, while the maximum concentration of DON in feed was 751 μg/kg. Besides, 3 and 15ADON were only detected in 10% of the serum samples (max. 1.3 ng/mL). Sulphate conjugates of DON were only detected in a few serum samples. Qualitative screening for phase II metabolites of other mycotoxins showed similar results. Overall, correlations between feed and serum concentrations of all mycotoxins were lacking (R²= 0.18 for DON).

由于欧洲真菌毒素的最大指导值仅可用于饲料，因此动物中真菌毒素的暴露主要通过饲料分析进行监控。但是，由于饲料中霉菌毒素的分布不均匀和分布不均，这可能会妨碍对动物体内霉菌毒素暴露的评估，因此需要适当的样品收集以确保获得可靠的结果。对比利时40个随机选择的肉鸡场进行了横断面研究。在对该动物年龄为28天的农场进行的一次访问中，收集了饲料线起点和终点的混合饲料样品。通过经过验证的多霉菌毒素LC-MS / MS方法分析饲料样品。此外，每个农场从10只随机选择的鸡中收集血清样本。血清中真菌毒素的浓度和主要体内定量分析了I期代谢物，同时通过UPLC-HRMS方法以定性方法确定了II期代谢物的存在。脱氧雪腐酚（DON）是最常见的霉菌毒素，在74％的饲料样品中存在，阳性样品中的平均浓度为270±171μg/ kg，最大浓度为751μg/ kg。一半的样品中也存在乙酰化的3-和15-乙酰基脱氧雪腐酚（3和15ADON），但是浓度较低（8±3μg3ADON和10±7μg15ADON / kg）。在平均血清浓度和标准偏差（SD）为11±19 ng / mL的血清样品中，仅在17.5％的农场中检测到DON。在饲喂被191μgDON / kg污染的日粮的肉鸡中，检测到的最高血清浓度为49 ng DON / mL，饲料中DON的最大浓度为751μg/ kg。此外，仅在10％的血清样品中检测到3和15ADON（最大1.3 ng / mL）。DON的硫酸盐结合物仅在少数血清样品中检测到。对其他真菌毒素的II期代谢产物进行定性筛选显示了相似的结果。总体而言，饲料中所有真菌毒素的浓度与血清浓度之间缺乏相关性（R² = DON 0.18）。