Lignocellulosic biomass (LB) is a cost-effective, readily available, and attractive substrate to produce 2,3-butanediol (2,3-BD) commercially. Fermentative conversion of non-detoxified wheat straw hydrolysate (WSH) to 2,3-BD was investigated using Paenibacillus polymyxa. The results show that P. polymyxa co-utilizes representative LB-derived sugars (glucose, xylose and arabinose). Comparatively, 2,3-BD fermentations with P. polymyxa in cultures containing glucose-xylose-arabinose in the ratio of 8:11:1 resulted in 1.03- and 1.18-fold increases in 2,3-BD yield and productivity, respectively, relative to glucose-only cultures (control). Subsequently, batch fermentations were conducted using 60 %, 80 %, and 100 % WSH and glucose-only control. P. polymyxa exhibited considerable tolerance to lignocellulose-derived microbial inhibitory compounds (LDMICs) in the 60 %, 80 % and 100 % WSH, as evidenced by growth, which increased 1.16-, 1.26- and 1.32-fold, respectively, when compared to the glucose-only control. The concentrations of 2,3-BD were 32, 31 and 23 g/L in 60 %, 80 % and 100 % WSH, respectively. These concentrations compare favorably to 32 g/L 2,3-BD produced with the glucose-only control without LDMICs. In addition, evaluation of the ability of P. polymyxa to transform LDMICs showed that P. polymyxa has the capacity to use HMF as a carbon source. These results underscore the potential of P. polymyxa for in situ detoxification and large-scale fermentation of WSH to 2,3-BD.

木质纤维素生物质（LB）是一种经济有效，易于获得且有吸引力的底物，可以商业化生产2,3-丁二醇（2,3-BD）。使用多粘芽孢杆菌研究了非排毒的麦草水解产物（WSH）向2,3-BD的发酵转化。结果表明，P。polymyxa共利用了代表性的LB衍生糖（葡萄糖，木糖和阿拉伯糖）。相比之下，在含有葡萄糖-木糖-阿拉伯糖比例为8：11：1的培养物中，用多粘多糖发酵的2,3-BD发酵分别导致2,3-BD产量和生产率分别提高1.03和1.18倍。相对于仅葡萄糖的培养物（对照）。随后，使用60％，80％和100％WSH和仅葡萄糖的对照进行分批发酵。如生长所证明，多粘体育假单胞菌对木质纤维素衍生的微生物抑制化合物（LDMIC）表现出相当高的耐受性，如60％，80％和100％的WSH所证明，与之相比分别增加了1.16、1.26和1.32倍。仅葡萄糖的控件。在60％，80％和100％的WSH中，2,3-BD的浓度分别为32、31和23 g / L。这些浓度与不使用LDMIC的纯葡萄糖对照所产生的32 g / L 2,3-BD相比具有优势。另外，对多粘多糖转化LDMIC的能力的评估表明，多粘多糖具有使用HMF作为碳源的能力。这些结果强调了潜在P.多粘了原位 WSH的解毒和大规模发酵成2,3-BD。