Abstract Background Vibrio species display variable and plastic fitness strategies to survive and interact with multiple hosts, including marine aquaculture species that are severely affected by pathogenic Vibrios. The culturable Vibrio sp. strain ArtGut-C1, the focus of this study, provides new evidence of such phenotypic plasticity as it accumulates polyhydroxybutyrate (PHB), a biodegradable polymer with anti-pathogen activity, particularly in the marine larviculture phase. The strain was isolated from the gut of laboratory-reared Artemia individuals, the live diet and PHB carrier used in larviculture. Its main phenotypic properties, taxonomic status and genomic properties are reported based on the whole-genome sequencing. Results Vibrio sp. ArtGut-C1 yielded 72.6% PHB of cells dry weight at 25°C. The genomic average nucleotide identity (ANI) shows it is closely related to V. diabolicus (ANI: 88.6%). Its genome contains 5,236,997-bp with 44.8% GC content, 3,710 protein-coding sequences, 96 RNA, 9 PHB genes functionally related to PHB metabolic pathways, and several genes linked to competing and colonizing abilities. Conclusions This culturable PHB-accumulating Vibrio strain shows high genomic and phenotypic variability. It may be used as a natural pathogen biocontrol in the marine hatchery and as a potential cell factory for PHB production.

中文翻译：

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弧菌属 ArtGut-C1，一种从水产养殖活饲料卤虫（甲壳纲）的肠道中分离出来的多羟基丁酸生产者

摘要 背景 弧菌物种显示出可变的和可塑性的适应策略以生存并与多种宿主相互作用，包括受致病弧菌严重影响的海水养殖物种。可培养的弧菌。本研究的重点菌株 ArtGut-C1 提供了这种表型可塑性的新证据，因为它积累了聚羟基丁酸酯 (PHB)，这是一种具有抗病原体活性的可生物降解聚合物，尤其是在海洋幼体养殖阶段。该菌株是从实验室饲养的卤虫个体的肠道、幼虫培养中使用的活饲料和 PHB 载体中分离出来的。其主要表型特性、分类状态和基因组特性是基于全基因组测序报告的。结果 弧菌 sp。ArtGut-C1 在 25°C 下产生 72.6% PHB 的细胞干重。基因组平均核苷酸同一性 (ANI) 表明它与 V. diabolicus (ANI: 88.6%) 密切相关。其基因组包含 5,236,997-bp，GC 含量为 44.8%，3,710 个蛋白质编码序列，96 个 RNA，9 个与 PHB 代谢途径功能相关的 PHB 基因，以及几个与竞争和定殖能力相关的基因。结论 这种可培养的 PHB 累积弧菌菌株显示出高度的基因组和表型变异性。它可用作海洋孵化场中的天然病原体生物防治和潜在的 PHB 生产细胞工厂。结论 这种可培养的 PHB 累积弧菌菌株显示出高度的基因组和表型变异性。它可用作海洋孵化场中的天然病原体生物防治和潜在的 PHB 生产细胞工厂。结论 这种可培养的 PHB 累积弧菌菌株显示出高度的基因组和表型变异性。它可用作海洋孵化场中的天然病原体生物防治和潜在的 PHB 生产细胞工厂。