Objective

To assess the effect of CPP-ACP/F recharging on ion release and hardness of GIC Fuji-Triage (VII) and Fuji-Triage-EP (VII-EP) containing CPP-ACP/F.

Methods

CPP-ACP distribution in Fuji-Triage-EP was determined using immunofluorescence. Thirty blocks of Fuji-Triage and Fuji-Triage-EP with the same surface area were placed individually in 5 mL of 50 mM lactic acid (pH 5) for three days. Every 12 h ten Fuji-Triage and ten Fuji-Triage-EP blocks were treated with 2 mL of either MI Paste Plus (CPP-ACP/F) solution (1 g paste + 4 mL water), Placebo MI paste solution (no CPP-ACP/F), or distilled water for 2 min. After each 2 min treatment the blocks were rinsed with distilled water and placed back into the acid. Calcium, inorganic phosphate and fluoride levels in the acid solution were measured using atomic absorption spectrophotometry, colorimetry and ion specific electrode respectively. Vickers surface hardness of the GIC was also determined. Data were analysed using a two-sample t-test and one-way ANOVA with a Bonferroni-Holm correction for multiple comparisons.

Results

CPP-ACP was distributed throughout Fuji-Triage-EP. Significantly (p < 0.001) higher calcium, inorganic phosphate and fluoride ion release and greater surface hardness (acid resistance) was observed in both GIC’s treated with the CPP-ACP/F paste. Fuji-Triage-EP released higher ion levels and exhibited greater surface hardness (acid resistance) than Fuji-Triage.

Significance

Topical application of CPP-ACP/F paste to GIC Fuji-Triage-EP recharged ion release and increased surface hardness (acid resistance) which may help improve properties and resistance to degradation as well as improve ion release for caries control.

中文翻译：

---

用CPP-ACP / F充填GIC并增加其硬度

目的

为了评估CPP-ACP / F充电对GIC含CPP-ACP / F的Fuji-Triage（VII）和Fuji-Triage-EP（VII-EP）的离子释放和硬度的影响。

方法

使用免疫荧光测定了Fuji-Triage-EP中的CPP-ACP分布。将三十块具有相同表面积的Fuji-Triage和Fuji-Triage-EP分别置于5 mL的50 mM乳酸（pH 5）中3天。每12小时，用2 mL的MI Paste Plus（CPP-ACP / F）溶液（1 g糊剂+ 4 mL水），安慰剂MI糊剂溶液（无CPP）处理十个Fuji-Triage和十个Fuji-Triage-EP嵌段。 -ACP / F）或蒸馏水2分钟。每处理2分钟后，将块用蒸馏水冲洗，然后放回酸中。分别使用原子吸收分光光度法，比色法和离子特异性电极测量酸溶液中的钙，无机磷酸盐和氟化物含量。还确定了GIC的维氏表面硬度。

结果

CPP-ACP已分发到整个Fuji-Triage-EP。在使用CPP-ACP / F糊剂处理的两种GIC中，均显着（p <0.001）更高的钙，无机磷酸盐和氟离子释放以及更高的表面硬度（耐酸性）。富士Triage-EP释放出更高的离子水平，并且比富士Triage表现出更高的表面硬度（耐酸性）。

意义

将CPP-ACP / F糊剂局部涂覆到GIC Fuji-Triage-EP上可使离子释放并增加表面硬度（耐酸性），这可能有助于改善性能和抗降解性，并改善控制龋齿的离子释放。