Laccase was immobilized on Electropositive granular media (EGM) consisting of Al(OH)₃ precipitate and silica gel through electrostatic adsorption to obtain Lac-EGM with high stability, sustainable recycling capacity, and outstanding environmental tolerance, which could be sealed in a polypropylene filter cartridge for convenient storage and industrial application. Lac-EGM was highly effective in decontaminating not only sarin (GB) and soman (GD), but also V-agents (VX), reaching a removal efficiency of nearly 100% within 30 min, and the toxicities of corresponding decontamination products were significantly reduced. It was the hydroxyl nucleophilic attack that contributed a lot in decontaminating nerve agents, with P-F bond fracture occurring in GB and GD degradation, and P-S bond fracture in VX degradation. Lac-EGM possessed both the inherent remarkable biotechnological applicability of laccase and nucleophilic attack advantages, which highlighted the potential value of nucleophilic attack reactions as a mechanism for the decontamination of nerve agents and broad prospects for water treatment application.

漆酶固定在由Al（OH）₃组成的正电颗粒介质（EGM）上通过静电吸附沉淀和硅胶，得到具有高稳定性，可持续的回收能力和出色的环境耐受性的Lac-EGM，可以将其密封在聚丙烯滤筒中，以方便储存和工业应用。Lac-EGM不仅对沙林（GB）和梭曼（GD），而且对V剂（VX）都具有很高的去污效果，在30分钟内达到近100％的去除效率，并且相应去污产品的毒性非常明显减少。羟基亲核攻击在净化神经毒剂中起了很大作用，在GB和GD降解中发生了PF键断裂，在VX降解中发生了PS键断裂。Lac-EGM具有漆酶固有的卓越生物技术适用性和亲核攻击优势，