Abstract Soil microbial communities have been used as indicators of changes in soil health agroecosystems. However, few studies have evaluated soil health under turfgrass systems especially in semiarid climates. Our study determined whether microbial biomass and composition in residential soils were controlled by home age along a turfgrass chronosequence and evaluated effects of turfgrass management in structuring soil microbial communities. Soil samples were obtained from nine locations within each home age category: oldest (1950–1970), middle (1971–1990), newer (1991–2010), and newest (2011–present) in summer 2018 and 2019. Soil microbial biomass and composition were assessed using chloroform fumigation extraction method (CFEM) and ester-linked fatty acid methyl ester (EL-FAME) analysis. Soil microbial biomass carbon (MBC) and nitrogen (MBN) were 46–52% and 65–75% higher in oldest homes when compared to newest homes, respectively. Neither total FAMEs nor fungal FAME abundance differed for home age categories, but bacterial FAME abundance increased with home age. Non-parametric analysis determined no microbial abundance differences with management practices or turfgrass species. Pearson correlations indicated soil organic matter and silt content most consistently altered the microbial community. Soil microbial communities within semiarid, urban environments shifted from high fungal to bacterial dominated as landscapes matured, potentially due to long-term effects of irrigation, fertilization, and pesticide use. Our results indicate time after establishment was more important to development of soil microbial communities in semiarid, perennial turfgrass systems than subtle differences in management, suggesting soil health and resource conservation goals in this setting may be achievable with relatively low levels of management over time.

中文翻译：

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半干旱气候下城市景观的土壤微生物生物量和组成

摘要 土壤微生物群落已被用作土壤健康农业生态系统变化的指标。然而，很少有研究评估草坪系统下的土壤健康，尤其是在半干旱气候下。我们的研究确定了住宅土壤中的微生物生物量和组成是否受家庭年龄沿草坪草时间序列的控制，并评估了草坪管理对构建土壤微生物群落的影响。2018 年和 2019 年夏季从每个家庭年龄类别中的九个地点获取土壤样本：最旧 (1950-1970)、中间 (1971-1990)、新 (1991-2010) 和最新 (2011-至今)。 土壤微生物生物量使用氯仿熏蒸提取法 (CFEM) 和酯连接的脂肪酸甲酯 (EL-FAME) 分析评估其成分和成分。与最新房屋相比，最旧房屋的土壤微生物生物量碳 (MBC) 和氮 (MBN) 分别高出 46-52% 和 65-75%。家庭年龄类别的 FAME 总量和真菌 FAME 丰度均无差异，但细菌 FAME 丰度随家庭年龄而增加。非参数分析确定微生物丰度与管理实践或草坪草种类没有差异。Pearson 相关性表明土壤有机质和淤泥含量最一致地改变了微生物群落。随着景观的成熟，半干旱城市环境中的土壤微生物群落从真菌为主转变为以细菌为主，这可能是由于灌溉、施肥和农药使用的长期影响。我们的结果表明，建立后的时间对半干旱地区土壤微生物群落的发展更为重要，