4-aminopyridine (4-AP) is a widely used drug that induces seizure activity in rodents, especially in rats, although there is no consensus in the literature on the dose to be used in mice. The aim of the present study was to investigate the effect of the intraperitoneal administration of 4-AP in two doses (4 and 10 mg/kg) in vivo. EEG, movement, and video recordings were made simultaneously in male B6 mice to specify the details of the seizures and to determine whether there is a suitable non-lethal dose for seizure induction and for further molecular studies. Seizure behavior in mice differs from that seen in rats, with no characteristic stages of epileptic seizures, but with spiking and seizure activity. Seizure activity, although produced at both doses without being lethal, induced different changes of the EEG pattern. Smaller dose induced a lower amplitude seizure activity, decreased spiking activity and later onset of seizures, while higher dose induced a much more intense brain seizure activity and severe trembling. It is concluded that the intraperitoneal administration of 4-AP at a dose of 10 mg/kg induces explicit seizure activity in mice which is repeatable and can be suitable for further molecular research.

4-氨基吡啶 (4-AP) 是一种广泛使用的药物，可在啮齿动物中诱发癫痫发作，尤其是在大鼠中，尽管文献中对用于小鼠的剂量尚未达成共识。本研究的目的是研究在体内以两种剂量（4 和 10 mg/kg）腹膜内给药 4-AP 的效果。在雄性 B6 小鼠中同时进行脑电图、运动和视频记录，以指定癫痫发作的细节，并确定是否有合适的非致死剂量用于癫痫诱导和进一步的分子研究。小鼠的癫痫发作行为与大鼠的不同，没有癫痫发作的特征阶段，但具有尖峰和癫痫发作活动。癫痫发作活动，虽然在两种剂量下都不会致命，但会引起脑电图模式的不同变化。较小的剂量引起较低幅度的癫痫发作活动，减少的尖峰活动和较晚的癫痫发作，而较高的剂量引起更强烈的脑癫痫发作活动和严重的颤抖。得出的结论是，以 10 mg/kg 的剂量腹膜内给药 4-AP 会在小鼠中诱导明确的癫痫发作活动，这是可重复的，可适用于进一步的分子研究。