A promising use of bismuth nanoparticles (BiNPs) for different biomedical applications leads to a search for the elucidation of their toxicity mechanisms, since toxicity studies are still at early stage. In the current study, cytotoxic effects of BiNPs produced by laser ablation in solution (LASiS) was investigated in the murine macrophage line RAW 264.7. The cells were exposed to 0.01–50 µg ml⁻¹ of BiNPs for 24 and 48 h and then cytotoxicity assays were performed. Decrease of MTT conversion to formazan and of cell attachment were observed with no effects on cell proliferation. No loss of membrane integrity or significant changes of ROS and RNS levels were observed in exposed cells. Foremost, increased phagocytic activity and DNA repair foci occurred for cells exposed to BiNPs. These effects are important findings that must be considered in the case of biomedical application of BiNPs, since inappropriate macrophages activation and inactivation may lead to immunotoxicity.

由于毒性研究仍处于早期阶段，铋纳米颗粒 (BiNPs) 在不同生物医学应用中的有前景的用途导致了对其毒性机制的阐明。在目前的研究中，在小鼠巨噬细胞系 RAW 264.7 中研究了溶液中激光烧蚀 (LASiS) 产生的 BiNPs 的细胞毒性作用。细胞暴露于 0.01–50 µg ml ^-1BiNPs 24 和 48 小时，然后进行细胞毒性测定。观察到 MTT 转化为甲臜和细胞附着减少，但对细胞增殖没有影响。在暴露的细胞中没有观察到膜完整性的丧失或 ROS 和 RNS 水平的显着变化。最重要的是，暴露于 BiNPs 的细胞发生了吞噬活性和 DNA 修复病灶的增加。这些影响是在 BiNPs 的生物医学应用中必须考虑的重要发现，因为不适当的巨噬细胞激活和失活可能导致免疫毒性。