Electrospun polymeric nanofibers as carriers for anticancer drugs have received a great deal of attention to treat tumor cells. This work was aimed to prepare an optimized nanofibrous sample based on poly(vinyl alcohol) (PVA)/chitosan (CS) blend, and then evaluate it containing 5-fluorouracil (5-FU) in terms of morphology, drug release, and cell culture. The electrospinning conditions to produce PVA/CS (50/50) blend nanofibers with an average diameter of approximately 150.8 nm were adjusted as follows: applied voltage 17 kV, needle tip to collector distance 60 cm, and flow rate 0.1 mL/h. The obtained results from Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) showed that there were no chemical interactions between the polymers and drug during the electrospinning process and the uniform morphology without beads. Moreover, to prolong 5-FU release from the blend nanofibers, three layered samples consisting of PVA/CS blend and poly (ε-caprolactone) (PCL) [PVA/CS-PCL 3-layers] were electrospun. On the other hand, by adding PCL in the PVA/CS blend nanofibers, the samples showed more hydrophobic property. Eventually, thiazolyl blue (MTT) assay along with NIH 3T3 cells culture proved that the sample could kill more than 80% of the cells. This formulation could be a promising candidate for cancer therapy potentially.

电纺聚合物纳米纤维作为抗癌药物的载体已受到治疗肿瘤细胞的广泛关注。这项工作旨在基于聚乙烯醇（PVA）/壳聚糖（CS）混合物制备优化的纳米纤维样品，然后评估其在形态，药物释放和细胞方面包含5-氟尿嘧啶（5-FU）的能力。文化。如下生产平均直径约为150.8 nm的PVA / CS（50/50）共混纳米纤维的静电纺丝条件如下：施加电压17 kV，针尖到收集器的距离为60 cm，流速为0.1 mL / h。傅里叶变换红外（FTIR）光谱和扫描电子显微镜（SEM）获得的结果表明，在静电纺丝过程中，聚合物和药物之间没有化学相互作用，并且没有珠粒，形态均匀。此外，为延长5-FU从共混纳米纤维中的释放，对由PVA / CS共混物和聚（ε-己内酯）（PCL）[PVA / CS-PCL 3层]组成的三层样品进行了静电纺丝。另一方面，通过在PVA / CS共混纳米纤维中添加PCL，样品显示出更多的疏水性。最终，噻唑基蓝（MTT）检测与NIH 3T3细胞培养一起证明该样品可以杀死80％以上的细胞。该制剂可能是潜在的癌症治疗的候选者。将由PVA / CS共混物和聚（ε-己内酯）（PCL）[PVA / CS-PCL 3层]组成的三层样品进行电纺。另一方面，通过在PVA / CS共混纳米纤维中添加PCL，样品显示出更多的疏水性。最终，噻唑基蓝（MTT）检测与NIH 3T3细胞培养一起证明该样品可以杀死80％以上的细胞。该制剂可能是潜在的癌症治疗的候选者。将由PVA / CS共混物和聚（ε-己内酯）（PCL）[PVA / CS-PCL 3层]组成的三层样品进行电纺。另一方面，通过在PVA / CS共混纳米纤维中添加PCL，样品显示出更多的疏水性。最终，噻唑基蓝（MTT）检测与NIH 3T3细胞培养一起证明该样品可以杀死80％以上的细胞。该制剂可能是潜在的癌症治疗的候选者。