Nutrient stress is the most important environmental stress that limits plant growth and development. Although recent evidence highlights the vital functions of long non-coding RNAs (lncRNA) in response to single nutrient stress in some model plants, a comprehensive investigation of the effect of lncRNAs in response to nutrient stress has not been performed in Arabidopsis thaliana. Here, we presented the identification and characterization of lncRNAs under seven nutrient stress conditions. The expression pattern analysis revealed that aberrant expression of lncRNAs is a stress-specific manner under nutrient stress conditions and that lncRNAs are more sensitive to nutrient stress than protein-coding genes (PCGs). Moreover, competing endogenous RNA (ceRNA) network and lncRNA-mRNA co-expression network (CEN) were constructed to explore the potential function of these lncRNAs under nutrient stress conditions. We further combined different expressed lncRNAs with ceRNA network and CEN to select key lncRNAs in response to nutrient stress. Together, our study provides important information for further insights into the role of lncRNAs in response to stress in plants.

营养胁迫是限制植物生长发育的最重要的环境胁迫。尽管最近的证据强调了长链非编码 RNA (lncRNA) 在某些模式植物中响应单一营养胁迫的重要功能，但尚未在拟南芥中对 lncRNA 响应营养胁迫的影响进行全面调查. 在这里，我们介绍了在七种营养胁迫条件下 lncRNA 的鉴定和表征。表达模式分析表明，lncRNAs的异常表达是营养胁迫条件下的一种胁迫特异性方式，并且lncRNAs对营养胁迫比蛋白质编码基因（PCGs）更敏感。此外，还构建了竞争性内源 RNA (ceRNA) 网络和 lncRNA-mRNA 共表达网络 (CEN)，以探索这些 lncRNA 在营养胁迫条件下的潜在功能。我们进一步将不同表达的 lncRNA 与 ceRNA 网络和 CEN 结合起来，选择响应营养胁迫的关键 lncRNA。总之，我们的研究为进一步了解 lncRNA 在植物应激反应中的作用提供了重要信息。