Abstract

The biomass production of Rhodovulum sulfidophilum HPB.6 was optimized via response surface methodology (RSM), and the optimal medium components such as waste soybean extract, yeast extract, and Mg²⁺ were determined using “one-single-factor-at-one-time” approach. RSM used a three-factor and central composite rotatable design consisting of 21 experimental runs conducted to optimize the final medium components. The optimized conditions were as follows: 2.723 g/L waste soybean extract, 3 g/L yeast extract, and 22 mg/L Mg²⁺. Under optimized conditions of Rhodovulum sulfidophilum HPB.6, the biomass production was 4.665 ± 0.326 g/L, which was 5.7-folds higher than that under non-optimized conditions. Besides that, the total lipid production was 5.7 times higher corresponding to the increase in biomass productivity. In addition, there was a change in total fatty acid composition with omega 7 and omega 9 which increased from 55.4 to 62.21 and from 3.4 to 9.41, respectively, while omega 6 decreased from 9.79 to 4.54 and omega 3 could not be detected. This exploration of waste soybean under optimized conditions would be a significant impact for the higher biomass production from Rhodovulum sulfidophilum HPB.6.

Graphic abstract

中文翻译：

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通过废大豆提取物可持续种植，以紫色非硫细菌生产更高的维甲酸

摘要

通过响应面法（RSM）优化了硫丹红球菌HPB.6的生物量生产，并使用“单因素合一”确定了最佳培养基成分，如废大豆提取物，酵母提取物和Mg ^2+。时间”的方法。RSM使用了三因素和中央复合旋转设计，包括21个实验运行，以优化最终培养基成分。优化条件如下：2.723 g / L废大豆提取物，3 g / L酵母提取物和22 mg / L Mg ²⁺。在优化条件下，嗜硫红球藻HPB.6的生物量产量为4.665±0.326 g / L，比非优化条件下高5.7倍。除此之外，总脂质产量高5.7倍，对应于生物质生产力的提高。此外，总脂肪酸组成也发生了变化，欧米茄7和欧米茄9分别从55.4增加到62.21和从3.4到9.41，而欧米茄6从9.79下降到4.54，并且无法检测到欧米茄3。在最佳条件下对废大豆的这种探索将对硫丹红球菌HPB.6产生更高的生物量产生重大影响。

图形摘要