Natural products are considered to be the lifeline treatment for several diseases where their structural complexity makes them a source of potential lead molecules. As a producer of antibiotics, food colorants, enzymes, and nutritious food, fungi are beneficial to humans. Fungi, as a source of novel natural products, draw attention of scientists. However, redundant isolation of metabolite retards the rate of discovery. So, apart from the standard conditions for the production of secondary metabolites, certain induction strategies are used to trigger biosynthetic genes in fungi. Advancement in the computational tools helps in connecting gene clusters and their metabolite production. Therefore, modern analytical tools and the genomic era in hand leads to the identification of manifold of cryptic metabolites. The cryptic biosynthetic gene cluster (BGC) has become a treasure hunt for new metabolites representing biosynthetic pathways, regulatory mechanisms, and other factors. This review includes the use of chemical inducers/epigenetic modifiers and co-culture (species interaction) techniques to induce these BGCs. Furthermore, it cites a detailed representation of molecules isolated using these strategies. Since the induction occurs on the genomic molecular DNA and histones, this together brings a significant exploration of the biosynthetic pathways.

Graphical Abstract

天然产物被认为是多种疾病的生命线治疗方法，它们的结构复杂性使其成为潜在铅分子的来源。作为抗生素，食用色素，酶和营养食品的生产者，真菌对人类有益。作为新型天然产物的真菌，引起了科学家的注意。但是，代谢物的多余分离会阻碍发现速度。因此，除了产生次级代谢产物的标准条件外，某些诱导策略还用于触发真菌中的生物合成基因。计算工具的进步有助于连接基因簇及其代谢产物。因此，现代的分析工具和手头的基因组时代导致了对隐性代谢产物的多种鉴定。隐秘的生物合成基因簇（BGC）已成为寻找代表生物合成途径，调节机制和其他因素的新代谢物的宝藏。这项审查包括使用化学诱导剂/表观遗传修饰剂和共培养（物种相互作用）技术来诱导这些BGC。此外，它引用了使用这些策略分离的分子的详细表示。由于诱导发生在基因组分子DNA和组蛋白上，因此一起带来了对生物合成途径的重大探索。它引用了使用这些策略分离的分子的详细表示。由于诱导发生在基因组分子DNA和组蛋白上，因此一起带来了对生物合成途径的重大探索。它引用了使用这些策略分离的分子的详细表示。由于诱导发生在基因组分子DNA和组蛋白上，因此一起带来了对生物合成途径的重大探索。

图形概要