The study examined the relationship between the contents of principal flavones of Scutellaria baicalensis Georgi and the activity of endogenous b-glucuronidase (sGUS) in differentiated and undifferentiated in vitro cultures grown on solid and liquid medium. Using RP-HPLC, it was shown that sGUS activity did not correlate with the increase in biomass of cultures, but depended on tissue differentiation. In hairy roots cultured both on liquid and solid medium, it was ten times higher than in undifferentiated cultures. At the same time, the share of aglycone forms in the total content of flavones during the culture cycle depended on the growing conditions. It was significantly higher on agar medium than on liquid medium. A correlation between sGUS activity and total aglycone content, as well as wogonin content, was observed only in hairy roots grown in the liquid medium.

研究考察了黄S中主要黄酮含量之间的关系。在固体和液体培养基上培养的分化和未分化体外培养物中，Georgi和内源性b-葡萄糖醛酸酶（sGUS）的活性。使用RP-HPLC，显示sGUS活性与培养物生物量的增加不相关，而是取决于组织分化。在液体和固体培养基上培养的毛状根中，其含量是未分化培养物中的十倍。同时，在培养周期中，苷元形式占黄酮总含量的比例取决于生长条件。在琼脂培养基上它明显高于在液体培养基上。仅在液体培养基中生长的毛状根中观察到了sGUS活性和总苷元含量以及沃戈宁含量之间的相关性。