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FELINE GIARDIASIS IN TURKEY: PREVALENCE AND GENETIC AND HAPLOTYPE DIVERSITY OF GIARDIA DUODENALIS BASED ON THE β-GIARDIN GENE SEQUENCE IN SYMPTOMATIC CATS
Journal of Parasitology ( IF 1.0 ) Pub Date : 2020-10-29 , DOI: 10.1645/19-183
Neslihan Sursal 1 , Emrah Simsek 2 , Kader Yildiz 3
Affiliation  

Giardia duodenalis is a common zoonotic protozoan parasite with a broad host distribution. The main objectives of the present study were to determine the prevalence of giardiasis and to reveal the genetic and haplotype diversity of G. duodenalis in symptomatic cats in Turkey. Fecal samples were collected from cats (n = 102) with diarrhea that were admitted to different pet clinics in the Central Anatolia region of Turkey. All samples were analyzed by microscopic examination (ME), rapid immunochromatographic test (ICT), and PCR targeting the β-giardin (bg) loci of the parasite. Phylogenetic, haplotype, and network analyses of G. duodenalis based on the bg gene were carried out. Overall, G. duodenalis was detected in 70/102 (68.6%) of the cats with diarrhea by ME (38/102, 37.3%), ICT (51/102, 50%), and PCR (30/102, 29.4%). According to sequence analyses of the bg gene region, all isolates were identified as G. duodenalis assemblage B. Haplotype analyses revealed 2 known and 8 novel haplotypes for G. duodenalis assemblage B. This study provides first prevalence and genetic and haplotype diversity data on G. duodenalis assemblage B from cats in Turkey.



中文翻译:

土耳其猫鞭毛虫病:基于症状猫的β-鞭毛虫基因序列,十二指肠鞭毛虫的患病率以及遗传和单倍型多样性

贾第鞭毛虫是一种常见的人畜共患的原生动物寄生虫,具有广泛的宿主分布。本研究的主要目的是确定贾第鞭毛虫病的患病率,并揭示土耳其有症状猫中十二指肠球菌的遗传和单倍型多样性。从腹泻的猫(n = 102)收集粪便样品,这些样品被送入土耳其中部安纳托利亚地区的不同宠物诊所。通过显微镜检查(ME),快速免疫色谱测试(ICT)和靶向寄生虫的β-贾第蛋白(bg)位点的PCR对所有样品进行了分析。进行了基于bg基因的十二指肠球菌的系统发育,单倍型和网络分析。总体而言,十二指肠球菌ME(38/102,37.3%),ICT(51/102,50%)和PCR(30/102,29.4%)在70/102(68.6%)腹泻的猫中被检出。据序列的分析BG基因区,所有分离被鉴定为G. duodenalis组合B.单倍型分析揭示2已知的,并且8点新颖的单倍型G. duodenalis组合B.本研究提供第一患病率和遗传和单倍型多样性数据ģ 。来自土耳其的duodenalis组合B。

更新日期:2020-10-30
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